FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_8.341000000098c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_8.341000000098c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329240
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT6870.2086623739521322No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC4480.13607095128173977No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATC4190.12726278702466287No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC4190.12726278702466287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.12422548900498118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGG207.0265296E-444.9992430
TCTAGGG250.002105103835.9993932
TCCCAGA300.0051424129.99949625
CGTATGC751.6952981E-929.99949541
GTATGCC751.6952981E-929.99949542
GTACTAA406.1300263E-428.1458991
TATGCCG803.3778633E-928.12452543
ATGCCGT803.3778633E-928.12452544
GCACGAC507.143973E-526.99954637
CTCGTAT856.4464984E-926.4701439
CCCACGA856.4464984E-926.4701419
TCCCGCA608.169431E-626.2495631
ATCGCCA608.169431E-626.2495637
CATGCTA1159.094947E-1225.4343514
GACAGCG801.0491931E-725.3120737
GCCCACG901.1847078E-824.99957818
GGCGAAT901.1847078E-824.99957832
CCCGGCT551.3654647E-424.5450415
CGAACTA1201.6370905E-1124.3745929
GTAACCC651.51966215E-524.2303611