FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_79.34100000009deb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_79.34100000009deb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018344
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC68810.6757048698671568No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT12670.12441768204064638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGG207.0342683E-444.9982344
TGCCGTC6450.040.81635745
AGGGGGT250.002107402535.9985855
ATGCCGT8050.032.70379344
TATGCCG8900.029.58039543
GCCCACG9150.029.01525518
CGAGACG9250.027.7286423
CCCACGA9700.027.37005819
CGAGCCC9800.027.32035415
CCGAGCC9900.027.04439414
CGTATGC9850.026.72483841
CTCGTAT10050.026.41687239
AGCCCAC10050.026.41687217
GAGCCCA10150.026.37827516
GTATGCC10050.026.19342
CCACGAG10250.025.9014220
ACGAGAC10350.025.8685522
GACGATA10150.025.4916126
TCCGAGC10550.025.37814913
TAACCTA10200.025.3666531
Produced by FastQC (version 0.11.2)