FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_78.34100000009dde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_78.34100000009dde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975871
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC60930.6243653105789597RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT13350.1368008681475318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6200.042.09543245
GCCCACG7850.033.2473518
CATTCCG7650.032.94012529
CCCACGA8050.032.7008219
ATGCCGT8000.032.62396244
CCGAGCC8150.032.5756514
CGAGCCC8100.032.49896215
TATGCCG8100.031.94342643
AGCCCAC8250.031.63535717
CCACGAG8400.031.07043620
CGTATGC8550.030.52534341
CGAGACC8450.030.08779723
TCCGAGC8750.030.08475513
ACGGGCC300.00514768429.99904316
TCCGAAT8500.029.9108132
CCGAATC8550.029.73589533
GAGCCCA8950.029.4124716
CACGAGA9200.029.3468921
GTATGCC8850.029.23635742
ACGAGAC9000.028.99907322
Produced by FastQC (version 0.11.2)