FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_77.34100000009dc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_77.34100000009dc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163739
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC34010.2922476603430838No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT12080.10380334422065429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4150.039.03495445
AGCCGTC300.005148054629.99908328
TGCGACC5100.029.11675829
ATGCCGT5650.029.06990844
TATGCCG5600.028.92768743
GCCCACG5550.028.78290418
CCCACGA5550.028.78290419
GACTGCG5250.028.28485126
GCGACCT5450.027.24687430
CGACCTA5500.026.99917631
CGTATGC6050.026.77604141
CCACGAG6050.026.77604120
CTGCGAC5550.026.7559428
GACCTAT5850.025.38383932
CCGAGCC6400.024.96017514
CGAGCCC6400.024.96017515
AGCCCAC6500.024.57617417
TATCTCG6350.024.4480736
ACGAGAC6950.024.27983322
AGACTGC6450.023.72020725
Produced by FastQC (version 0.11.2)