FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_76.34100000009db4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_76.34100000009db4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255110
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC27120.21607667853813609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4050.037.77747745
TCGCGCG458.704028E-734.9983259
TACCGCG708.1490725E-1032.141327
CGCGCGC501.9825493E-631.49849510
GCCCACG4750.031.26166318
ATGCCGT4900.030.7650644
TATGCCG4950.030.45430443
ATACGCG300.0051486429.99856815
CCCACGA5150.028.396719
AGCCCAC5300.028.01752717
CGAGCCC5400.027.49868615
ACCGCGA903.965397E-1027.4986848
CCGAGCC5450.027.24640514
CGTATGC5650.027.07835241
GTATGCC5650.026.6801442
GCGCCAT608.195373E-626.24874721
CGAGACC4950.025.4533323
GAGCCCA6200.023.95046816
GTACCGC1152.6557245E-1023.4771376
CCACGAG6250.023.39888220
Produced by FastQC (version 0.11.2)