FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_75.34100000009da8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_75.34100000009da8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237173
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC82650.6680553164351307No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT24970.20183111011960334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10100.040.98851445
CAGCCCC250.002107570435.99860812
ATGCCGT11550.035.84277344
GCGCGCT702.1827873E-1135.35577811
TATGCCG12450.033.43244643
GCCCACG12650.033.25958618
GTATGCC12500.033.1187242
CCCACGA12850.032.74192419
CGTATGC13100.031.94533341
AGCCCAC13250.031.92329617
CGCGCTA501.9823783E-631.49878312
CTGCGCG501.9823783E-631.4987839
CGAGACT12750.031.41055323
CCACGAG13500.031.16546220
CCGAGCC13550.030.88441714
TCGTATG13600.030.77086840
ACGAGAC13750.030.43518822
AGACTAG12700.030.29410625
CGAGCCC13900.030.26861615
TCGCGCG300.00514838129.9988429
Produced by FastQC (version 0.11.2)