FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_74.34100000009d9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_74.34100000009d9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469748
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10340.22011802072600628TruSeq Adapter, Index 12 (95% over 21bp)
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG8340.17754200124321975No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC7360.15667975169665438No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC5760.12261893611042517No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC5560.1183613341621465No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5040.10729156909662202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2650.032.26254739
CCCACGA2650.032.26254719
TATGCCG2700.031.66846543
GCCCACG2700.031.66509618
GTATGCC2700.031.66509642
TCTCGTA2850.030.78794538
CGTATGC2850.029.9985141
TCGTATG2850.029.9985140
ATGCCGT2900.029.48443444
CGAGACT2550.029.11620123
CACGAGA3050.028.03139521
ACGAGAC3100.027.57927522
ACGTCCC1550.026.12773324
CGTCCCG1550.026.12773325
CTCCGAG3450.025.4335212
GTCCCGG1600.025.31124126
CCACGTC1600.025.31124122
GCCACGT1600.025.31124121
CGCCACG1600.025.31124120
TAACCGG1350.025.0014244
Produced by FastQC (version 0.11.2)