FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_73.34100000009d71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_73.34100000009d71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC825.183312262958281No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC613.8558786346396965No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC513.2237673830594185No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC311.9595448798988624No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT140.8849557522123894No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT110.695322376738306No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT70.4424778761061947No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC50.31605562579013907No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG40.2528445006321113No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG30.18963337547408343No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGGATCTCGTATGCCGTC30.18963337547408343No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC30.18963337547408343TruSeq Adapter, Index 12 (95% over 21bp)
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTG20.12642225031605564No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT20.12642225031605564No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAG20.12642225031605564No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG20.12642225031605564No Hit
GGTTATCAATGACAGATTTGGAATTGTTGAGGGTCTTATGACTACAGTCCA20.12642225031605564No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG20.12642225031605564No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA20.12642225031605564No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGCATCTCGTATGCCGTC20.12642225031605564No Hit
CTTATACACATCCCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC20.12642225031605564RNA PCR Primer, Index 44 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTT20.12642225031605564No Hit
TCTTATACAATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC20.12642225031605564No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC252.36841E-545.045
CCTATCT300.003732798929.99999835
CTATCTC300.003732798929.99999836
TCTTATA350.00794357625.7142851
AGCCCAC457.5744407E-425.017
GTATGCC457.5744407E-425.042
ATCTCGT457.5744407E-425.037
GCCCACG457.5744407E-425.018
CCACGAG457.5744407E-425.020
TATGCCG457.5744407E-425.043
GAGCCCA457.5744407E-425.016
CACGAGA457.5744407E-425.021
CCCACGA457.5744407E-425.019
TCTCGTA457.5744407E-425.038
ACGAGAC457.5744407E-425.022
ATGCCGT457.5744407E-425.044
CGTATGC457.5744407E-425.041
CGAGCCC457.5744407E-425.015
CCGAGCC457.5744407E-425.014
TCGTATG457.5744407E-425.040
Produced by FastQC (version 0.11.2)