FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_72.34100000009d64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_72.34100000009d64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159524
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.17677590832727363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.11408941601263761No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.11283568616634487No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1610.10092525262656402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG302.1541327E-644.99874543
GCCCACG356.215707E-638.5703518
GTATGCC356.215707E-638.5703542
ATGCCGT356.215707E-638.5703544
CCGGCAC301.135706E-437.49895520
CGTATGC401.5524836E-533.7490641
GCGGTCG401.5524836E-533.7490616
ACCTAGG401.5524836E-533.7490630
AAACCTA401.5524836E-533.7490628
GGCGGTC401.5524836E-533.7490615
TCGTATG401.5524836E-533.7490640
CGGTCGC401.5524836E-533.7490617
TCTCGTA352.8100298E-432.1419638
GTCCCCC352.8100298E-432.1419611
CGTCCCC352.8100298E-432.1419610
AAGCGCC453.4728833E-529.99916619
CCCACGA453.4728833E-529.99916619
AACCTAG453.4728833E-529.99916629
CTAGGGC453.4728833E-529.99916632
CACGCGG300.00513483229.99916324
Produced by FastQC (version 0.11.2)