FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_71.34100000009d58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_71.34100000009d58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274254
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC270002.1188868153445077No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT66690.5233650433900934No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA20860.1637036258077275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG20840.16354667122881308No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT18770.14730187231117187No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC18580.14581080381148498No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG18550.14557537194311337No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG17590.1380415521552218No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG16730.13129250526190225No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA12820.1006078850841355No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA207.034785E-444.9984340
TGCCGTC29150.043.3792645
AGCCGTG250.002107555335.9987457
TCCGGGG250.002107555335.99874513
ATGCCGT36500.034.70562744
CCGTTAC4050.032.824291
TATGCCG38800.032.6483443
CGTATGC39700.032.0769741
CGAGACA38700.031.91749223
GTATGCC39700.031.90694842
TCGTATG40100.031.86921340
CTCGTAT40100.031.81310539
TATCTCG39400.031.80726436
ACGAGAC40350.031.72751622
TCTCGTA40500.031.27668838
CCACGAG40800.031.2672920
TCGCGCG651.4042598E-831.1527589
ATAATAT39300.031.14395332
CCCACGA41000.031.00501819
CACGAGA41200.030.90911521
Produced by FastQC (version 0.11.2)