FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_70.34100000009d4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_70.34100000009d4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379828
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC67680.490495916882394No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT15230.11037607585873022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8000.038.81218745
ATGCCGT9300.033.3868344
GCCCACG9550.032.74724218
TATGCCG9500.032.6839543
CCCACGA10050.031.11802519
AGCCCAC10200.030.66040817
CCGAGCC10550.029.85649914
CGAGACT10550.029.64323823
GTATGCC10750.029.09173442
GACTTGC10600.029.07890326
CGAGCCC10850.029.03097215
GAGCCCA11200.028.32463816
CTCGTAT11350.027.55384639
CGTATGC11300.027.47665841
TCCGAGC11600.027.34792513
CCACGAG11700.026.92188620
GCTGGAA11300.026.8793431
CTCCGAG12100.025.84596312
TGCTGGA12050.025.5797830
TTGCTGG12100.025.28813629
Produced by FastQC (version 0.11.2)