FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_7.341000000098ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_7.341000000098ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831897
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG300.00514772729.998489
TACCGCG2050.028.5351377
ACCGCGA2000.028.1235718
GACCCCC507.1585346E-526.9986298
CGGGCAG709.3064773E-725.71298217
GTCCGGG709.3064773E-725.71298231
GTACCGC2300.025.433496
CGAGGTA2300.025.4334912
GCGAGGT2250.024.9987311
CCCGAGC551.36823E-424.5442112
CCGGGCA751.6974573E-623.99878133
CCGTTAC2000.023.6693271
TGCCGTC1550.023.22741945
CGCGAGG2500.022.4988610
GGGTCCG903.2862772E-722.49885729
CCGGCAC500.002264755822.49885720
CCCACGA1500.022.49885719
CGGGCAC802.974426E-622.49885734
GGGCACG802.974426E-622.49885735
GGCTGCG500.002264755822.4988577
Produced by FastQC (version 0.11.2)