FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_69.34100000009d3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_69.34100000009d3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591120
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC158070.9934511539041682No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT29740.18691236361808033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC18150.043.14049545
ATGCCGT21500.036.41860644
CGCTATA21100.036.04152331
TATGCCG22150.035.4514743
ACGACGC21500.035.37098327
GCCCACG22350.035.3344618
CGACGCT21600.035.20722628
CGAGACG21800.034.98743423
CCCACGA22850.034.6597419
GACGCTA22000.034.56709729
GTATGCC22900.034.38756642
CGTATGC23000.034.23805241
TATCTCG23100.033.9924436
CGAGCCC23400.033.6527915
CCACGAG23500.033.60532820
AGCCCAC23600.033.46293317
CCGAGCC23700.033.22680714
TCGTATG24050.032.83680740
GACGACG23350.032.66492526
ACGCTAT23250.032.61187730
Produced by FastQC (version 0.11.2)