FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_68.34100000009d21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_68.34100000009d21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204364
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC43140.3581973556167405No Hit
CTTCTATTCGAAGTGCTTTTCACCTTTCCTTCACAGTACTTGTTCACTATC13240.1099335416867326No Hit
GTTTCAGGTTCTATTTCACCCTTCTATTCGAAGTGCTTTTCACCTTTCCTT12550.10420437674988625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6250.041.03848345
GCCCACG7400.035.26896718
CCCACGA7700.033.8948519
CGAGCCC7800.033.46030415
CCGAGCC7850.033.2471814
ATGCCGT7750.033.0955544
AGCCCAC7900.033.03675517
CAAGGCG6950.032.69663629
TCCGAGC8200.031.82809313
GAGCCCA8200.031.82809316
GGCGAAT7100.031.6889732
TATGCCG8100.031.66549943
GTATGCC8300.031.17354842
CGTATGC8400.031.07028441
GCGAATC7500.030.29888233
AGGCGAA7450.030.20022631
ACGAGAC8700.029.99889422
CTCGTAT8900.029.5775639
CTCCGAG8900.029.5775612
AAGGCGA7700.029.51189830
Produced by FastQC (version 0.11.2)