FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_67.34100000009d14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_67.34100000009d14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345526
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC110120.818415994934323No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT24580.18267948742722176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12500.041.7602145
ATGCCGT15600.033.46170444
GCCCACG15950.033.0083418
CCCACGA16150.032.73888419
CGAGCCC16100.032.7008115
TATGCCG16000.032.6251643
CCGAGCC16150.032.59956714
ACCGTGC352.8211644E-432.1418238
AGCCCAC16500.031.90806417
GTATGCC16500.031.63534542
GAGCCCA16650.031.62060416
CCACGAG16900.031.28597520
TAACCTA16300.031.19531431
CGAGACG16600.030.90261823
ACCTATC16700.030.852333
AACCTAT16750.030.35723132
CGTATGC17350.030.08548541
CGATAAC17400.029.4818128
ACGATAA17250.029.4773127
TATCTCG17900.029.4124636
Produced by FastQC (version 0.11.2)