FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_66.34100000009d07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_66.34100000009d07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268111
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC20940.1651275006683169RNA PCR Primer, Index 46 (95% over 21bp)
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA12810.10101639367531706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2850.040.262118
CCCACGA2950.038.8972819
TATGCCG3050.036.1465943
CCGAGCC3250.035.99905414
TGCCGTC3200.034.4522245
CGAGACC2950.033.5584423
CGTATGC3300.033.40821541
ATGCCGT3300.033.40821544
CTCCGAG3600.031.87416312
GTATGCC3600.031.2491842
CGAGCCC3750.030.59919515
CCACGAG3850.029.80441320
TCCGAGC4100.028.53583713
AGCCCAC4050.028.3325917
CTGCGCG406.167447E-428.1242629
CAGTGCC406.167447E-428.1242629
ACGAGAC4400.026.59020822
CTCGTAT4350.026.37861839
TCGTATG4400.026.0788640
GAGCCCA4400.026.0788616
Produced by FastQC (version 0.11.2)