FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_65.34100000009cf9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_65.34100000009cf9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882583
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC853729.672971267291574No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT192722.183590665127246No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG69810.7909737667732101No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCT12720.1441224224803786No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGG11340.12848649928675265No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT8980.10174680454982704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC88350.044.5677945
ATGCCGT109250.036.0417844
TATGCCG116600.033.78914643
CGTATGC116900.033.7197741
TATCTCG117150.033.59019536
CACGAGA117550.033.55245621
GACTGCG116950.033.5322126
CCCACGA117300.033.5280619
GCGACCT116900.033.52730630
GTATGCC117600.033.5190642
GACCTAT117050.033.50356332
CGACCTA117200.033.47987731
CCACGAG117550.033.47589520
GCCCACG117500.033.47099318
ACGAGAC117950.033.43866722
ATCTCGT117750.033.43814537
CGAGACT117650.033.42831823
AGACTGC117500.033.41354825
CTCGTAT117850.033.4097739
TGCGACC117400.033.4036829
Produced by FastQC (version 0.11.2)