FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_64.34100000009cec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_64.34100000009cec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634245
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC32320.19776716465401456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4600.036.19434745
GTCGCGC352.8214912E-432.1416978
GCCCACG5550.030.40431218
GGTCGCG300.00514879329.9989197
CCGAGCC5950.028.36032114
CGAGCCC5950.028.36032115
TATGCCG6100.027.66293743
ATGCCGT6050.027.51966944
CCCACGA6550.026.1059319
AGCCCAC6500.025.96060217
CGTATGC6450.025.81302541
TCCGAGC6650.025.7133613
GTATGCC6500.025.61446242
TACCGCG1152.6557245E-1023.4774137
CCACGAG7400.023.10727520
GAGCCCA7350.022.95835716
TCGTATG7300.022.80739640
CTCCGAG7550.022.64819112
GTACCGC1305.0931703E-1122.499196
CGCGAGG1204.5292836E-1022.4991910
Produced by FastQC (version 0.11.2)