FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_63.34100000009cdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_63.34100000009cdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035159
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC51250.49509302435664476No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC12180.11766308364222308No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT10900.1052978334729254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7650.042.35107845
CTGCGAC1650.040.9072928
TGCGACC1650.040.9072929
GCGACCT1750.038.56973330
CGACCTA1750.038.56973331
TATGCCG8850.036.86278543
ATGCCGT8950.036.4509144
ACTGCGA1800.036.248427
CGTATGC9050.036.04813441
ACGAGAC8950.035.6967522
CGAGACT8250.035.45298423
GACTGCG1850.035.2687226
CCACGAG9150.034.91649620
CTCGTAT9400.034.70591739
CACGAGA9300.034.35332521
TCTCGTA9650.034.0399538
TATCTCG9500.033.8669336
ATCTCGT9700.033.8644937
CCCACGA9500.033.63009619
GTATGCC9750.033.46006842
Produced by FastQC (version 0.11.2)