FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_62.34100000009cc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_62.34100000009cc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808400
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC9830.12159821870361208No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC8360.10341415141019297TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3350.033.58070443
GCCCACG3450.033.259518
CTCGTAT3650.031.4370639
CCCACGA3650.031.4370619
TAGGGCC300.00514740329.9987645
ATGCCGT3850.029.80396744
GTATGCC3900.028.84496542
CCGAGCC4000.028.1238414
CGTATGC4200.026.78460941
GACTGCG1850.026.75565526
TATCTCG2950.026.69381536
ACGAGAC4300.026.68494622
CTCCGAG4400.026.58981112
TCTCGTA4450.025.78545438
TGCCGTC4450.025.78545445
TCGTATG4450.025.27985840
ATCTCGT4800.024.84272837
GGTATCA4500.024.5445331
ACTGCGA2200.024.54444127
TGCGACC2050.024.14534629
Produced by FastQC (version 0.11.2)