FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_61.34100000009ca9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_61.34100000009ca9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545835
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC12060.2209458902415565RNA PCR Primer, Index 27 (95% over 21bp)
GCTTTAGATTATCCTTGAAAACTTCGAACCTAAGAAACTTCTCTTCAACGG6450.1181675781142653No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6430.11780116701933735No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5900.10809127300374655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.10570960088671486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2550.036.1812643
TGCGACC458.692259E-734.9982229
GTATGCC2750.033.54374742
CGAGACG2150.033.48666823
GCCCACG2900.032.5845518
ATGCCGT2850.032.37270444
CCCACGA2950.032.0322719
CGTATGC2900.031.80872541
ATCTCGT3050.030.98202937
CACGAGA3150.030.71272321
TCGTATG3100.030.48231940
TATCTCG3050.030.24436236
TGCCGTC3150.030.00397145
TTAGGCG453.488732E-529.9984724
ACTGCGA602.416109E-729.99847227
CGACCGT300.00514619829.99847210
CTGCGAC602.416109E-729.99847228
CTCGTAT3150.029.28422439
TCTCGTA3300.028.63490738
GACTGCG554.1613184E-628.63490726
Produced by FastQC (version 0.11.2)