FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_60.34100000009c9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_60.34100000009c9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862752
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC12560.1455806535365899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1850.036.4870543
CCCACGA1850.036.48493619
GCCCACG1900.035.52480718
ATGCCGT2050.032.92733844
ACCCTGG2050.031.82791527
CCGAGCC2400.029.99872414
AGCCCAC2350.029.67958817
GAGACCC2200.029.65783124
CGTCCCG406.166421E-428.12380425
CGTATGC2550.027.3517841
CGAGACC2500.026.99885223
CGAGCCC2700.026.66553515
TGCCGTC2550.026.47099745
CCTGGAT2500.026.0988929
GTATGCC2600.025.96043442
ACGAGAC2600.025.96043422
CTCGTAT2700.025.83223739
CTCCGAG2900.025.6023612
CCCTGGA2550.025.58714928
TCTCGTA2750.025.36255838
Produced by FastQC (version 0.11.2)