FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_6.34100000009883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_6.34100000009883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811442
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC13160.1621804146198989RNA PCR Primer, Index 46 (95% over 21bp)
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA8400.10351941358716951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2250.032.9986943
GCCCACG2400.031.87373418
ATGCCGT2350.031.5944944
CGTATGC2450.030.30491841
CGTCCCG602.4183464E-729.99880825
CGAGCCC2700.028.33220915
CGAGACC2700.028.33220923
TGCCGTC2700.027.49890945
CCCACGA2750.026.99892819
CCGAGCC2750.026.99892814
CACGAGA3100.026.12799521
GTCCCGG709.305113E-725.71326426
GTATGCC2900.025.60243242
CTCCGAG3000.025.49898712
ATTCCGA2950.025.16849130
CGCCACG751.6972081E-623.99904620
TCCGAGC3100.023.9506613
AGCCCAC3150.023.57049217
AATCTCG3250.023.53752536
ACGAGAC3250.023.53752522
Produced by FastQC (version 0.11.2)