FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_59.34100000009c8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_59.34100000009c8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239828
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC54370.4385285700919805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7350.038.57032445
ATGCCGT8100.034.72122644
TATGCCG9300.030.48299643
CGTATGC9750.028.84532741
GTATGCC10050.028.43201842
ATAATAT10000.027.89919932
CCCACGA10100.027.84573719
CTCGTAT10200.027.79332439
GCCCACG10050.027.76039718
TCGTATG10150.027.70856540
TATCTCG10350.027.60790436
CGAGACA10100.027.40020623
CCACGAG10350.026.95574820
CACGAGA10950.025.4787221
GCCCCTA801.0542317E-725.31177327
AGCCCAC11100.025.13441317
ACGAGAC11100.025.13441322
ACGGGCT450.001226814324.9992833
CGAGCCC11200.024.9115
TCTCGTA11500.024.65146838
Produced by FastQC (version 0.11.2)