FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_58.34100000009c72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_58.34100000009c72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232807
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC82620.6701778948367425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9850.039.97472445
ATGCCGT11600.033.94405744
TATGCCG11700.033.6539443
GCCCACG12200.032.6422118
CCCACGA12500.032.03878419
GTATGCC12450.031.62530942
CGTATGC12750.031.05764841
CGAGACT12900.030.69651223
TCGTATG13000.030.63345540
ACGAGAC13150.030.28402522
CTCGTAT13400.029.88692939
GACTTGC13400.029.88692926
CCACGAG13350.029.83033220
AGCCCAC13400.029.71902517
CACGAGA13650.029.1747221
TCTCGTA14150.028.30281638
GAGCCCA14600.027.27636316
CGAGCCC14900.026.72717715
TTGCTGG14750.026.69390329
CCGAGCC15150.026.28613314
Produced by FastQC (version 0.11.2)