FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_57.34100000009c65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_57.34100000009c65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC221.28130460104834No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC181.04834012813046No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC140.8153756552125802No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC120.6988934187536401No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT50.29120559114735No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA50.29120559114735No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT40.23296447291788003No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC30.17472335468841002No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG30.17472335468841002No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT30.17472335468841002No Hit
GACGTTACCTCACAGCCTCTGCTATTTTCAGAGGACAGATGAGCACAAAAG20.11648223645894001No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC20.11648223645894001No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG20.11648223645894001No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT20.11648223645894001No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG20.11648223645894001No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.11648223645894001TruSeq Adapter, Index 12 (95% over 21bp)
GTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTT20.11648223645894001No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT20.11648223645894001No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT20.11648223645894001No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT20.11648223645894001No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC20.11648223645894001RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)