FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_56.34100000009c59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_56.34100000009c59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928166
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC76640.8257143657492302No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT14740.1588077994669057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8800.037.33003645
ATGCCGT9250.035.5139844
GCCCACG9600.033.9829918
TATGCCG10050.032.68699643
AGCCCAC10100.032.30066317
CCCACGA10100.032.30066319
CCACGAG10150.032.1415520
CGTATGC10250.032.04747441
GGCGAAT10200.031.98399232
CAAGGCG10300.031.6734729
GCGAATC10350.031.52045633
CGAGCCC10350.031.52045615
TCGTATG10450.031.43412640
CCGAGCC10350.031.30307414
CTCGTAT10550.031.13617339
GTATGCC10550.031.13617342
GAGCCCA10600.030.77704616
TCTCGTA10700.030.69968438
AGGCGAA10750.030.13830831
GCACGCC300.00514777929.99877724
Produced by FastQC (version 0.11.2)