FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_53.34100000009c22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_53.34100000009c22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222144
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC54160.4431556346878927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6400.039.72681845
ATGCCGT7350.034.59205644
GCCCACG7750.032.51500718
GCGACCT7350.032.14174730
CTGCGAC7300.032.05368828
CGACCTA7350.031.83563431
CCCACGA7950.031.69701619
CCTATCT8100.031.11003534
TGCGACC7600.031.08445429
TATGCCG8200.031.00629443
AGCCCAC8200.031.00502617
TATCTCG8150.030.91917836
TCGTATG8250.030.81711640
CGAGCCC8250.030.54439715
GACCTAT7750.030.48281932
ACCTATC7800.030.28741333
CGTATGC8400.030.2668141
CCGAGCC8450.030.08771714
GGGCGGT300.005148253429.99896618
CTCGTAT8500.029.9107339
Produced by FastQC (version 0.11.2)