FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_52.34100000009c15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_52.34100000009c15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276977
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC139251.0904659989960663No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT22130.17329991064835154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC16550.041.7377445
ATGCCGT18850.036.883844
TATGCCG19800.035.1141343
CGTATGC20150.034.5028541
TCGTATG20300.034.24790640
GCCCACG20750.033.8304718
CCCACGA20900.033.5876719
GTATGCC20800.033.42463742
CTCGTAT20750.033.39674839
CGAGACC21050.033.02766823
ACGAGAC21500.032.75498622
AGCCCAC21700.032.34941517
ACCTTGC21500.032.33639527
CCACGAG21800.032.20102320
CCTTGCA21800.031.99460428
CACGAGA22250.031.75200821
CCGAGCC22100.031.56029114
TCTCGTA21950.031.46845238
CGAGCCC22550.031.03025815
CTTGCAG22700.030.9243329
Produced by FastQC (version 0.11.2)