FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_51.34100000009c08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_51.34100000009c08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908163
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC63950.7041687450380603No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA22860.25171692746786645No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA22420.24687198223226448No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG17820.19622028204188016No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG17190.18928320136363186No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG14400.1585618440742466No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT10830.1192517202308396No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG10370.11418655021180119No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG9990.11000227932650858No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC9640.10614834561637063No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG9220.1015236251642051No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG9200.10130340038076865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8000.040.77985445
ACCGTCG250.002107230735.9987688
ATGCCGT9500.034.3409344
GCCCACG10550.030.7098518
CCACGAG10900.029.72375120
TATGCCG11000.029.65807543
GTATGCC11050.029.52387642
CGTATGC11100.029.39088641
AGCCCAC11050.029.32026317
TCGTATG11150.029.057340
CTAACGC2250.028.9990083
ATCCTAT10900.028.8980932
CTCGTAT11300.028.87069339
CGAGACT11300.028.87069323
CCCACGA11250.028.79901319
TCTCGTA11600.027.93007938
CGAGCCC11800.027.64735815
CCTATCT11400.027.43327134
TATCTCG11500.027.39036636
GAGCCCA11900.027.22595816
Produced by FastQC (version 0.11.2)