FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_50.34100000009bfa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_50.34100000009bfa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788677
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC18070.2291178771537651TruSeq Adapter, Index 12 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3700.038.30924241
TGCCGTC3700.038.30924245
ATGCCGT3850.036.8166844
TATGCCG3950.036.45420543
AGCCCAC3900.035.7677717
GAGCCCA3900.035.7677716
CTCCGAG4000.035.43605412
CCGAGCC4000.035.43605414
CGAGCCC4000.035.43605415
CGAGACT4000.035.43605423
TCCGAGC4050.034.9985713
GCCCACG4000.034.87357718
CCCACGA4000.034.87357719
TCGTATG4200.033.7486240
GTATGCC4250.033.35157842
CTCGTAT4350.032.58487739
ACGAGAC4350.032.58487722
CACGAGA4350.032.06765721
CCACGAG4350.032.06765720
CTACTTG3850.030.97276328
Produced by FastQC (version 0.11.2)