FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_5.34100000009869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_5.34100000009869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663730
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA70831.0671507992707878No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT65160.9817244964066715No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA51290.7727539812875718No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG38210.5756858963735254No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT36120.5441971886158528No Hit
GTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCT22430.33793861961942356No Hit
ACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAA21400.3224202612508098No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTGTCTCTTATAC16580.24980037063263677No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC16090.2424178506320341No Hit
GTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGCTGTCTCTTATAC15290.23036475675349916No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGACTGTCTCT14450.2177090081810375No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA14340.2160517077727389No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTACTGTCTCT13270.19993069471019842No Hit
AGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGCTGTCTCTTAT11570.17431787021831166No Hit
GTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTT11040.16633269552378227No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCTGTCTCTTAT10590.15955283021710634No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACTGTCTC9900.14915703674686995No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGACTGTCTC9780.14734907266508973No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG9420.141925180419749No Hit
GCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTACTG7790.11736700164223404No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTC7660.1154083738869721No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATCTGTCTC7570.11405240082563693No Hit
AAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACC7310.11013514531511308No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCCTG7220.1087791722537779No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG7140.1075738628659244No Hit
ATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTACTGTCTC7020.10576589878414416No Hit
ATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTC6960.10486191674325403No Hit
CCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACCTGTCTCTT6780.10214997062058367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAC6850.037.8125271
CACTGTG300.00514642729.99914428
GGGGCAA1001.8189894E-1229.24916618
GAGTTTA15900.029.1808911
CTGCGAC2000.028.12419928
AGTTTAA16600.027.920892
GGTACGA1053.6379788E-1227.85635221
AGACTGC2050.027.43824425
CCCACGA2050.027.43824419
GACTGCG2050.027.43824426
TCTACGT507.155307E-526.999232
ATGCCGT2050.026.342744
TGCCGTC2050.026.342745
GTTCGTA3200.026.042351
TATGCCG2100.025.71549243
GGGCAAG1159.094947E-1225.43405719
GTATGCC2150.025.11556242
TCGTATG2150.025.11556240
ACGTTTG450.001226211824.999295
TGCGACC2250.024.9992929
Produced by FastQC (version 0.11.2)