FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_49.34100000009bd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_49.34100000009bd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505753
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG11420.22580192307312066No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC8340.16490263033536134No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC6510.12871895965026406No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA6140.12140313552267609No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGC6010.11883271082919925No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT5700.11270323656013903No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT5650.11171461167803255No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA5600.11072598679592606No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA5400.10677148726750015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG300.005145339329.9990775
GCCCACG1001.8189894E-1229.249118
CGTATGC952.3646862E-1128.42017741
TCGTCTA450.001225824524.9992319
CCCACGA1201.6370905E-1124.3742519
GTATAAG851.8647188E-723.8558141
ACCTATA2650.023.8058031
ATACCCG2650.023.7728545
TACCCGG2600.023.3646666
GCGCTAA1750.023.14214313
CTCGTAT1204.5110937E-1022.49930839
ATTAGAC500.00226327522.4993083
TATGCCG1204.5110937E-1022.49930843
TAACCTA1950.021.92240317
GCCTCGA2800.021.6957632
GGGGCAA2700.021.66599818
GCGCGCG1358.367351E-1121.6659989
TCGTATG1257.4942363E-1021.59933740
GGTATCA3050.021.4224341
GTATCAA7900.021.1045111
Produced by FastQC (version 0.11.2)