FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_48.34100000009bc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_48.34100000009bc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407486
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC13250.3251645455303986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.16074171873389514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.10282561854885813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2950.032.79589518
CCCACGA2950.032.79589519
GTATGCC2950.032.79589542
TATGCCG3000.032.24929443
ACGAGAC3000.032.24929422
ATGCCGT3050.031.72062144
CGTATGC3100.031.20899641
CTCGTAT3150.030.71361439
TCTCGTA3200.030.23371538
TCGTATG3200.030.23371540
CACGAGA3250.029.76858321
TGCCGTC3450.028.04286645
CGAGACC2700.027.49939723
ATCTCGT3750.026.99941337
AGCCCAC3750.025.79943817
CCGAGCC3750.025.79943814
CCTGGAT2900.025.60288829
TGGATAA3100.025.40267231
CTGGATA3200.025.31194730
ATAGAGC450.001225351724.9994546
Produced by FastQC (version 0.11.2)