FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_47.34100000009bb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_47.34100000009bb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420739
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC79300.5581602250659692No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT21680.15259664160693834No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA16630.11705175968281295No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT14450.10170763243635883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10100.040.09772545
CGCGCGC708.1490725E-1032.14182310
ATGCCGT12750.031.94015344
TATGCCG12800.031.81538443
CGTATGC13400.030.72662541
GCCCACG14000.029.5704818
CCCACGA14000.029.5704819
GTATGCC13850.029.56583842
TCGTATG14000.029.40977140
ACGAGAC14300.028.79278222
CCACGAG14450.028.64960120
CGAGACA12350.028.6023223
CTCGTAT14350.028.53567139
CACGAGA14550.028.45269821
GACGCTA1600.028.12409629
TATCTCG14400.027.96785436
TCTCGTA15150.027.02883738
TCGCGCG856.486516E-926.469749
CGAGACG1800.026.24915923
AGCCCAC16050.025.93374817
Produced by FastQC (version 0.11.2)