FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_46.34100000009baa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_46.34100000009baa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267858
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC312502.4647870660594484No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT97880.7720107456828762No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC25890.20420267884889318No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC23460.1850364946232149No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC20100.1585351040889437No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC18140.14307595961061884No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA17340.1367661047215067No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG16700.13171822081021692No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA15960.12588160503778814No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA13150.10371823973978159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC33200.043.30621745
GCGGTCC250.0021074835.9989934
ATGCCGT43400.033.1282644
GTCCGGC900.032.53631
TATGCCG45650.031.59400743
CGTATGC45600.031.57806241
CCACGAG46250.031.28020520
TCGTATG46300.031.19782840
GTATGCC46350.031.11563142
ACGAGAC46500.031.11203222
CCCACGA46700.030.97878819
CTCGTAT46650.030.96376439
CGAGACT46150.030.95796223
GACTTGC46050.030.92747326
CACGAGA46850.030.83158121
TCTCGTA46900.030.79870838
ATCTCGT47400.030.42636337
AGACTTG46900.030.36695125
GCTGGAA47000.030.30234131
TGCTGGA47100.030.19023730
Produced by FastQC (version 0.11.2)