FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_45.34100000009b9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_45.34100000009b9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208392
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC812056.720087521267932No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT113670.9406715701527318No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA90830.7516600573323888No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA86080.7123516209971599No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG68970.5707584955875246No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG65890.545270077921734No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG52500.4344616647577938No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG50380.41691768896186004No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC42550.3521208349608405No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC41330.3420247734178975No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG36480.30188879105455846No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG35490.2936960853762686No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG34890.28873080920760813No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC34120.2823587047911605No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC34080.2820276863799165No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT32290.26721461247674594No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT31080.2572013055366139No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT30050.24867758144708008No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT29440.24362955067560857No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA29160.24131242179690035No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA28140.2328714523101775No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT28040.23204390628206742No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT26630.2203755072857152No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG24870.20581069719097775No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG24280.2009281756251283No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC23130.1914113963018623No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC21750.17999126111394317No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC21720.17974299730551013No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC21690.1794947334970771No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA21400.17709485001555786No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA20600.1704744817906772No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC20450.16923316274851208No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA19770.1636058497573635No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC19760.1635230951545525No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA19250.15930261041119106No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA18630.15417182503690854No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA18560.1535925428172315No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC17870.14788247522327191No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC17840.1476342114148389No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCTC17480.1446550457136426No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC16880.1396897695449821No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC16540.1368761130494078No Hit
CGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC16360.13538653019880964No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC16190.13397970195102252No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC16140.13356592893696748No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC15610.12917993498798402No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC15490.12818687975425191No Hit
CGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT15470.12802137054862991No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA15420.12760759753457487No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCTGTCTCTTATACACATCTC15290.12653178769803178No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA15010.12421465881932355No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACAT14600.1208217201040722No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC14570.12057345629563915No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT14330.11858734582817496No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC13620.11271176902859337No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT13470.11147044998642823No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA13110.10849128428523193No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTCC12660.10476732715873657No Hit
ATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG12620.10443630874749253No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATC12500.10344325351376044No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA12420.10278121669127238No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTCC12280.10162265225191824No Hit
TTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC12150.10054684241537515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC85500.044.00052645
ATGCCGT98050.038.39158644
TATCTCG101300.037.22496436
CGCTATA101800.037.21894531
GCTATAT101850.037.1785832
GACGCTA102100.037.13162229
TATGCCG101500.037.10881843
ACGAGAC102400.037.08875322
CTATATC101800.037.08633433
ACGACGC102100.037.0655127
CGACGCT102250.037.0331428
CCACGAG102600.036.97259520
GACGACG102300.036.9710526
ATATCTC102000.036.969535
ACGCTAT102550.036.96868530
CGAGACG102450.036.9388823
CTCGTAT102200.036.9191739
GTATGCC102100.036.91125542
AGACGAC102550.036.90286625
GAGACGA102550.036.90286624
Produced by FastQC (version 0.11.2)