FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_44.34100000009b80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_44.34100000009b80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251833
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC12530.10009322329735675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2700.033.33179518
CCCACGA2900.031.80887619
TATGCCG3050.030.24692343
ATGCCGT3250.028.38557244
GTATGCC3300.027.95325542
TGCCGTC3300.027.27364745
CCGAGCC3400.027.131114
AGCCCAC3450.026.73789417
CGTATGC3550.026.6184941
GACGCTA1251.8189894E-1225.19883529
CAAGGCG2300.023.47717529
CGAGACC2500.023.3989223
CCACGAG3850.023.37554420
GGCGAAT2350.022.97766132
CTCGTAT4250.022.76365739
GTCGCGC802.9762978E-622.498968
TCGTATG4550.021.75723840
CGACGCT1459.094947E-1221.72313528
CGAGCCC4250.021.70488215
CTCCGAG4150.021.68574712
Produced by FastQC (version 0.11.2)