FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_43.34100000009b73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_43.34100000009b73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC62626.36899747262005No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT582.443133951137321No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC562.358887952822241No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT190.8003369839932603No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC170.7160909856781803No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC120.5054759898904801No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG80.33698399326032014No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTTC60.2527379949452401Illumina PCR Primer Index 11 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT50.21061499578770007No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT40.16849199663016007Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC40.16849199663016007RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT30.12636899747262004No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA30.12636899747262004No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTAGATCTCGTATGCCGTC30.12636899747262004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC900.045.00000445
GTATGCC950.042.6315842
ATCTCGT950.042.6315837
GCCCACG950.042.6315818
CCACGAG950.042.6315820
TATGCCG950.042.6315843
CACGAGA950.042.6315821
CCCACGA950.042.6315819
TCTCGTA950.042.6315838
ACGAGAC950.042.6315822
ATGCCGT950.042.6315844
CGAGCCC950.042.6315815
CCGAGCC950.042.6315814
TCGTATG950.042.6315840
CTCGTAT950.042.6315839
CTATATC900.042.50000433
ACGCTAT900.042.50000430
TATATCT900.042.50000434
CGCTATA900.042.50000431
GCTATAT900.042.50000432