##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23V3BCXX l01n01 kb2_1_42.34100000009b66.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.56758832565284	38.0	32.0	38.0	32.0	38.0
2	35.16282642089094	38.0	32.0	38.0	32.0	38.0
3	35.20122887864823	38.0	32.0	38.0	32.0	38.0
4	35.30030721966206	38.0	32.0	38.0	32.0	38.0
5	35.57142857142857	38.0	32.0	38.0	32.0	38.0
6	37.05222734254993	38.0	38.0	40.0	32.0	40.0
7	36.6221198156682	38.0	38.0	40.0	32.0	40.0
8	36.59907834101382	38.0	38.0	40.0	32.0	40.0
9	36.69585253456221	38.0	38.0	40.0	32.0	40.0
10	36.56605222734255	38.0	38.0	40.0	32.0	40.0
11	36.92780337941628	38.0	38.0	40.0	32.0	40.0
12	36.26881720430107	38.0	38.0	40.0	32.0	40.0
13	36.66205837173579	38.0	38.0	40.0	32.0	40.0
14	36.28341013824885	38.0	38.0	40.0	32.0	40.0
15	36.22887864823349	38.0	38.0	40.0	32.0	40.0
16	36.18663594470046	38.0	38.0	40.0	32.0	40.0
17	35.795698924731184	38.0	38.0	40.0	27.0	40.0
18	35.84024577572965	38.0	38.0	40.0	27.0	40.0
19	35.93087557603687	38.0	38.0	40.0	27.0	40.0
20	35.935483870967744	38.0	38.0	40.0	27.0	40.0
21	36.32258064516129	38.0	38.0	40.0	32.0	40.0
22	36.43010752688172	38.0	38.0	40.0	32.0	40.0
23	36.26804915514593	38.0	38.0	40.0	32.0	40.0
24	36.11136712749616	38.0	38.0	40.0	27.0	40.0
25	35.97926267281106	38.0	38.0	40.0	27.0	40.0
26	35.92319508448541	38.0	38.0	40.0	27.0	40.0
27	35.51920122887865	38.0	38.0	40.0	27.0	40.0
28	35.67204301075269	38.0	32.0	40.0	27.0	40.0
29	35.62980030721966	38.0	32.0	40.0	27.0	40.0
30	35.904761904761905	38.0	38.0	40.0	27.0	40.0
31	36.05299539170507	38.0	38.0	40.0	27.0	40.0
32	35.74347158218126	38.0	38.0	40.0	27.0	40.0
33	35.8863287250384	38.0	38.0	40.0	27.0	40.0
34	35.8678955453149	38.0	38.0	40.0	27.0	40.0
35	35.87864823348694	38.0	38.0	40.0	27.0	40.0
36	36.08909370199693	38.0	38.0	40.0	27.0	40.0
37	35.763440860215056	38.0	38.0	40.0	27.0	40.0
38	35.8863287250384	38.0	38.0	40.0	27.0	40.0
39	35.17818740399385	38.0	32.0	40.0	27.0	40.0
40	35.663594470046085	38.0	38.0	40.0	27.0	40.0
41	35.784178187403995	38.0	38.0	40.0	27.0	40.0
42	35.68049155145929	38.0	38.0	40.0	27.0	40.0
43	35.966973886328724	38.0	38.0	40.0	27.0	40.0
44	35.70046082949309	38.0	38.0	40.0	27.0	40.0
45	35.94162826420891	38.0	38.0	40.0	27.0	40.0
46	35.42089093701997	38.0	38.0	40.0	27.0	40.0
47	35.36098310291859	38.0	32.0	40.0	27.0	40.0
48	35.28110599078341	38.0	32.0	40.0	27.0	40.0
49	35.45238095238095	38.0	32.0	40.0	27.0	40.0
50	35.355606758832565	38.0	38.0	40.0	27.0	40.0
51	34.62903225806452	38.0	32.0	38.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5423280423280374
1101	2	2.949735449735442
1101	3	3.092592592592588
1101	4	-0.09788359788360168
1101	5	-1.857142857142854
1101	6	2.470899470899461
1101	7	0.19576719576718915
1101	8	1.9417989417989432
1101	9	2.0052910052909994
1101	10	1.4126984126984112
1101	11	-1.1560846560846514
1101	12	-0.15873015873015817
1101	13	-0.3068783068783105
1101	14	0.26719576719577276
1101	15	1.8835978835978864
1101	16	1.7566137566137527
1101	17	2.6560846560846514
1101	18	1.187830687830683
1101	19	2.2089947089947017
1101	20	2.7857142857142847
1101	21	2.558201058201057
1101	22	1.716931216931215
1101	23	1.6190476190476133
1101	24	1.55555555555555
1101	25	0.6084656084656004
1101	26	-5.7645502645502695
1101	27	1.9444444444444429
1101	28	-0.08994708994709555
1101	29	2.574074074074069
1101	30	-7.584656084656078
1101	31	0.4021164021163983
1101	32	1.9682539682539684
1101	33	3.3148148148148096
1101	34	0.46825396825396126
1101	35	-7.253968253968257
1101	36	-1.3624338624338606
1101	37	-0.16137566137566495
1101	38	-5.1428571428571495
1101	39	3.4867724867724874
1101	40	1.6164021164021136
1101	41	-5.031746031746035
1101	42	-0.812169312169317
1101	43	-5.45238095238096
1101	44	-0.9603174603174622
1101	45	1.6746031746031633
1101	46	-5.415343915343922
1101	47	-6.137566137566143
1101	48	-1.3597883597883609
1101	49	2.978835978835974
1101	50	-5.690476190476193
1101	51	3.283068783068778
2201	1	2.5423280423280374
2201	2	-0.050264550264557784
2201	3	0.09259259259258812
2201	4	-1.0978835978836017
2201	5	2.142857142857146
2201	6	2.1375661375661252
2201	7	2.529100529100525
2201	8	-0.7248677248677211
2201	9	1.6719576719576636
2201	10	-1.253968253968253
2201	11	1.8439153439153486
2201	12	-1.4920634920634939
2201	13	-0.9735449735449748
2201	14	-2.8994708994708915
2201	15	2.216931216931222
2201	16	-10.410052910052912
2201	17	-0.6772486772486843
2201	18	2.187830687830683
2201	19	3.2089947089947017
2201	20	4.11904761904762
2201	21	3.8915343915343925
2201	22	3.0502645502645507
2201	23	1.952380952380949
2201	24	1.8888888888888857
2201	25	2.941798941798936
2201	26	2.9021164021163983
2201	27	1.2777777777777786
2201	28	4.24338624338624
2201	29	2.9074074074074048
2201	30	3.0820105820105894
2201	31	3.735449735449734
2201	32	3.301587301587304
2201	33	2.981481481481474
2201	34	2.801587301587297
2201	35	2.4126984126984112
2201	36	2.3042328042328037
2201	37	3.838624338624335
2201	38	5.190476190476183
2201	39	4.820105820105823
2201	40	3.9497354497354493
2201	41	0.6349206349206327
2201	42	-2.312169312169317
2201	43	3.214285714285708
2201	44	-2.4603174603174622
2201	45	2.007936507936499
2201	46	0.2513227513227463
2201	47	0.19576719576718915
2201	48	-9.193121693121697
2201	49	-9.18783068783069
2201	50	-1.0238095238095255
2201	51	1.9497354497354422
2202	1	-3.4576719576719626
2202	2	2.949735449735442
2202	3	1.0925925925925881
2202	4	1.9021164021163983
2202	5	-8.190476190476186
2202	6	-0.19576719576721047
2202	7	2.195767195767189
2202	8	2.6084656084656146
2202	9	1.338624338624328
2202	10	1.4126984126984112
2202	11	1.510582010582013
2202	12	2.507936507936506
2202	13	2.359788359788361
2202	14	0.9338624338624442
2202	15	0.5502645502645578
2202	16	-5.910052910052912
2202	17	-6.343915343915352
2202	18	-5.812169312169317
2202	19	1.5423280423280374
2202	20	-0.2142857142857153
2202	21	2.558201058201057
2202	22	-0.283068783068785
2202	23	-2.047619047619051
2202	24	0.22222222222222143
2202	25	-0.3915343915343996
2202	26	1.235449735449734
2202	27	3.944444444444443
2202	28	4.576719576719576
2202	29	2.574074074074069
2202	30	0.7486772486772537
2202	31	3.0687830687830697
2202	32	0.6349206349206398
2202	33	1.3148148148148096
2202	34	1.1349206349206327
2202	35	3.746031746031747
2202	36	2.970899470899468
2202	37	2.5052910052909994
2202	38	3.1904761904761827
2202	39	0.8201058201058231
2202	40	1.6164021164021136
2202	41	3.301587301587297
2202	42	0.8544973544973473
2202	43	1.547619047619044
2202	44	-0.29365079365079794
2202	45	0.34126984126983473
2202	46	0.9179894179894106
2202	47	2.8624338624338534
2202	48	1.6402116402116391
2202	49	0.9788359788359742
2202	50	-1.0238095238095255
2202	51	-7.716931216931226
1102	1	-7.791005291005295
1102	2	0.9497354497354422
1102	3	1.0925925925925881
1102	4	1.9021164021163983
1102	5	2.142857142857146
1102	6	3.1375661375661252
1102	7	0.8624338624338606
1102	8	2.6084656084656146
1102	9	0.005291005290999351
1102	10	-0.5873015873015888
1102	11	1.510582010582013
1102	12	1.8412698412698418
1102	13	2.359788359788361
1102	14	2.2671957671957728
1102	15	2.550264550264558
1102	16	1.7566137566137527
1102	17	1.98941798941798
1102	18	2.5211640211640187
1102	19	3.5423280423280374
1102	20	2.7857142857142847
1102	21	3.224867724867728
1102	22	1.716931216931215
1102	23	1.6190476190476133
1102	24	2.2222222222222214
1102	25	2.6084656084656004
1102	26	2.5687830687830626
1102	27	3.944444444444443
1102	28	1.9100529100529045
1102	29	2.574074074074069
1102	30	2.7486772486772537
1102	31	2.4021164021163983
1102	32	0.6349206349206398
1102	33	2.648148148148138
1102	34	-0.19841269841270304
1102	35	2.4126984126984112
1102	36	2.970899470899468
1102	37	3.1719576719576708
1102	38	4.523809523809518
1102	39	3.4867724867724874
1102	40	4.283068783068785
1102	41	3.9682539682539684
1102	42	3.5211640211640187
1102	43	3.547619047619044
1102	44	1.3730158730158735
1102	45	2.3412698412698347
1102	46	3.584656084656082
1102	47	3.529100529100525
1102	48	3.640211640211639
1102	49	3.6455026455026456
1102	50	2.642857142857139
1102	51	1.9497354497354422
2203	1	-3.4576719576719626
2203	2	2.949735449735442
2203	3	0.09259259259258812
2203	4	-1.0978835978836017
2203	5	-0.8571428571428541
2203	6	2.1375661375661252
2203	7	1.5291005291005249
2203	8	3.275132275132279
2203	9	2.6719576719576636
2203	10	1.746031746031747
2203	11	0.8439153439153486
2203	12	1.5079365079365061
2203	13	2.026455026455025
2203	14	-0.39947089947089154
2203	15	2.216931216931222
2203	16	1.0899470899470884
2203	17	2.3227513227513157
2203	18	1.187830687830683
2203	19	2.2089947089947017
2203	20	3.1190476190476204
2203	21	2.8915343915343925
2203	22	2.0502645502645507
2203	23	0.952380952380949
2203	24	1.8888888888888857
2203	25	1.941798941798936
2203	26	1.9021164021163983
2203	27	4.277777777777779
2203	28	4.24338624338624
2203	29	3.9074074074074048
2203	30	4.082010582010589
2203	31	2.735449735449734
2203	32	2.301587301587304
2203	33	2.981481481481474
2203	34	2.801587301587297
2203	35	2.4126984126984112
2203	36	-10.195767195767196
2203	37	-3.661375661375665
2203	38	3.1904761904761827
2203	39	-0.1798941798941769
2203	40	-0.05026455026455068
2203	41	2.6349206349206327
2203	42	-0.812169312169317
2203	43	2.214285714285708
2203	44	2.0396825396825378
2203	45	-0.992063492063501
2203	46	-2.2486772486772537
2203	47	3.195767195767189
2203	48	3.3068783068783034
2203	49	2.31216931216931
2203	50	2.9761904761904745
2203	51	-1.0502645502645578
1103	1	2.5423280423280374
1103	2	2.949735449735442
1103	3	0.09259259259258812
1103	4	-1.0978835978836017
1103	5	-0.8571428571428541
1103	6	-0.8624338624338748
1103	7	-0.47089947089947515
1103	8	2.275132275132279
1103	9	-2.3280423280423364
1103	10	0.7460317460317469
1103	11	0.8439153439153486
1103	12	-4.992063492063494
1103	13	1.0264550264550252
1103	14	1.6005291005291085
1103	15	1.216931216931222
1103	16	1.0899470899470884
1103	17	-1.6772486772486843
1103	18	2.187830687830683
1103	19	3.2089947089947017
1103	20	2.1190476190476204
1103	21	1.8915343915343925
1103	22	1.0502645502645507
1103	23	-2.047619047619051
1103	24	-2.1111111111111143
1103	25	-4.058201058201064
1103	26	1.9021164021163983
1103	27	-9.222222222222221
1103	28	-2.25661375661376
1103	29	-6.592592592592595
1103	30	-0.9179894179894106
1103	31	-4.264550264550266
1103	32	1.301587301587304
1103	33	1.9814814814814738
1103	34	2.801587301587297
1103	35	3.4126984126984112
1103	36	2.3042328042328037
1103	37	1.838624338624335
1103	38	3.1904761904761827
1103	39	2.820105820105823
1103	40	-9.55026455026455
1103	41	-0.36507936507936734
1103	42	-0.812169312169317
1103	43	2.214285714285708
1103	44	0.039682539682537765
1103	45	1.007936507936499
1103	46	-0.7486772486772537
1103	47	2.195767195767189
1103	48	0.3068783068783034
1103	49	-0.6878306878306901
1103	50	1.9761904761904745
1103	51	-2.550264550264558
2204	1	-0.45767195767196256
2204	2	2.949735449735442
2204	3	0.09259259259258812
2204	4	1.9021164021163983
2204	5	2.142857142857146
2204	6	2.1375661375661252
2204	7	-10.970899470899475
2204	8	-4.224867724867721
2204	9	-7.828042328042336
2204	10	-4.753968253968253
2204	11	0.8439153439153486
2204	12	-2.492063492063494
2204	13	-1.9735449735449748
2204	14	1.6005291005291085
2204	15	-4.283068783068778
2204	16	-10.410052910052912
2204	17	-1.6772486772486843
2204	18	1.187830687830683
2204	19	3.2089947089947017
2204	20	0.1190476190476204
2204	21	1.8915343915343925
2204	22	2.0502645502645507
2204	23	-2.047619047619051
2204	24	-2.1111111111111143
2204	25	-3.558201058201064
2204	26	-9.597883597883602
2204	27	-2.2222222222222214
2204	28	-2.25661375661376
2204	29	2.9074074074074048
2204	30	3.0820105820105894
2204	31	-10.764550264550266
2204	32	-4.198412698412696
2204	33	-10.518518518518526
2204	34	-2.698412698412703
2204	35	-3.0873015873015888
2204	36	-3.1957671957671963
2204	37	-10.661375661375665
2204	38	-9.309523809523817
2204	39	-22.179894179894177
2204	40	-0.05026455026455068
2204	41	2.6349206349206327
2204	42	3.187830687830683
2204	43	-2.285714285714292
2204	44	0.039682539682537765
2204	45	-1.992063492063501
2204	46	-10.248677248677254
2204	47	-10.30423280423281
2204	48	-13.193121693121697
2204	49	-10.18783068783069
2204	50	-4.0238095238095255
2204	51	-3.550264550264558
2205	1	0.5423280423280374
2205	2	0.9497354497354422
2205	3	3.092592592592588
2205	4	-0.09788359788360168
2205	5	2.142857142857146
2205	6	1.8042328042327895
2205	7	2.195767195767189
2205	8	1.9417989417989432
2205	9	2.0052910052909994
2205	10	0.7460317460317469
2205	11	1.510582010582013
2205	12	1.1746031746031704
2205	13	1.6931216931216895
2205	14	0.26719576719577276
2205	15	1.8835978835978864
2205	16	-0.9100529100529116
2205	17	1.3227513227513157
2205	18	1.8544973544973473
2205	19	-6.12433862433863
2205	20	0.1190476190476204
2205	21	-5.775132275132275
2205	22	-1.9497354497354493
2205	23	0.2857142857142847
2205	24	-6.777777777777782
2205	25	0.6084656084656004
2205	26	2.5687830687830626
2205	27	3.944444444444443
2205	28	0.24338624338624015
2205	29	-4.759259259259263
2205	30	2.7486772486772537
2205	31	0.4021164021163983
2205	32	-0.03174603174603163
2205	33	0.6481481481481381
2205	34	2.4682539682539613
2205	35	3.0793650793650755
2205	36	2.970899470899468
2205	37	-1.161375661375665
2205	38	-3.8095238095238173
2205	39	-0.1798941798941769
2205	40	1.6164021164021136
2205	41	3.301587301587297
2205	42	-1.1455026455026527
2205	43	0.2142857142857082
2205	44	2.706349206349202
2205	45	1.007936507936499
2205	46	2.2513227513227463
2205	47	0.8624338624338534
2205	48	2.9735449735449677
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1209	1	2.5423280423280374
1209	2	-0.050264550264557784
1209	3	0.09259259259258812
1209	4	-1.0978835978836017
1209	5	2.142857142857146
1209	6	1.1375661375661252
1209	7	3.529100529100525
1209	8	-10.224867724867721
1209	9	-3.8280423280423364
1209	10	1.746031746031747
1209	11	1.8439153439153486
1209	12	1.5079365079365061
1209	13	2.026455026455025
1209	14	-0.39947089947089154
1209	15	2.216931216931222
1209	16	2.0899470899470884
1209	17	2.3227513227513157
1209	18	3.187830687830683
1209	19	4.208994708994702
1209	20	4.11904761904762
1209	21	2.8915343915343925
1209	22	3.0502645502645507
1209	23	1.952380952380949
1209	24	2.8888888888888857
1209	25	3.941798941798936
1209	26	3.9021164021163983
1209	27	4.277777777777779
1209	28	4.24338624338624
1209	29	2.9074074074074048
1209	30	3.0820105820105894
1209	31	2.735449735449734
1209	32	2.301587301587304
1209	33	2.981481481481474
1209	34	3.801587301587297
1209	35	3.4126984126984112
1209	36	4.304232804232804
1209	37	3.838624338624335
1209	38	4.190476190476183
1209	39	2.820105820105823
1209	40	2.9497354497354493
1209	41	3.6349206349206327
1209	42	3.187830687830683
1209	43	3.214285714285708
1209	44	3.0396825396825378
1209	45	2.007936507936499
1209	46	3.2513227513227463
1209	47	2.195767195767189
1209	48	3.3068783068783034
1209	49	3.31216931216931
1209	50	3.9761904761904745
1209	51	3.949735449735442
1214	1	-0.45767195767196256
1214	2	-0.050264550264557784
1214	3	3.092592592592588
1214	4	-1.0978835978836017
1214	5	-0.8571428571428541
1214	6	-4.362433862433875
1214	7	-3.970899470899475
1214	8	-4.224867724867721
1214	9	0.6719576719576636
1214	10	-2.253968253968253
1214	11	0.8439153439153486
1214	12	0.5079365079365061
1214	13	-4.973544973544975
1214	14	-1.3994708994708915
1214	15	1.216931216931222
1214	16	-4.910052910052912
1214	17	1.3227513227513157
1214	18	-11.312169312169317
1214	19	-0.7910052910052983
1214	20	-13.38095238095238
1214	21	-6.6084656084656075
1214	22	-4.449735449735449
1214	23	0.952380952380949
1214	24	0.8888888888888857
1214	25	-10.558201058201064
1214	26	1.9021164021163983
1214	27	-9.222222222222221
1214	28	3.24338624338624
1214	29	1.9074074074074048
1214	30	-0.9179894179894106
1214	31	1.735449735449734
1214	32	-1.698412698412696
1214	33	-3.518518518518526
1214	34	-3.698412698412703
1214	35	-3.0873015873015888
1214	36	-3.1957671957671963
1214	37	2.838624338624335
1214	38	-8.309523809523817
1214	39	2.820105820105823
1214	40	-2.5502645502645507
1214	41	0.6349206349206327
1214	42	0.18783068783068302
1214	43	2.214285714285708
1214	44	-0.9603174603174622
1214	45	1.007936507936499
1214	46	-3.7486772486772537
1214	47	-0.8042328042328108
1214	48	-10.193121693121697
1214	49	2.31216931216931
1214	50	2.9761904761904745
1214	51	1.9497354497354422
1215	1	2.5423280423280374
1215	2	2.949735449735442
1215	3	-2.907407407407412
1215	4	1.9021164021163983
1215	5	-3.857142857142854
1215	6	1.1375661375661252
1215	7	1.5291005291005249
1215	8	-4.724867724867721
1215	9	-10.328042328042336
1215	10	0.7460317460317469
1215	11	-24.15608465608465
1215	12	-5.492063492063494
1215	13	-4.973544973544975
1215	14	-4.3994708994708915
1215	15	1.216931216931222
1215	16	1.0899470899470884
1215	17	-4.677248677248684
1215	18	1.187830687830683
1215	19	2.2089947089947017
1215	20	2.1190476190476204
1215	21	1.8915343915343925
1215	22	1.0502645502645507
1215	23	0.952380952380949
1215	24	-5.111111111111114
1215	25	-23.058201058201064
1215	26	1.9021164021163983
1215	27	-7.722222222222221
1215	28	-21.75661375661376
1215	29	-9.092592592592595
1215	30	-8.91798941798941
1215	31	-9.264550264550266
1215	32	1.301587301587304
1215	33	-4.018518518518526
1215	34	-4.198412698412703
1215	35	-3.5873015873015888
1215	36	-8.695767195767196
1215	37	-9.161375661375665
1215	38	-2.8095238095238173
1215	39	2.820105820105823
1215	40	2.9497354497354493
1215	41	-3.3650793650793673
1215	42	2.187830687830683
1215	43	4.214285714285708
1215	44	2.0396825396825378
1215	45	1.007936507936499
1215	46	-3.7486772486772537
1215	47	2.195767195767189
1215	48	2.3068783068783034
1215	49	-3.68783068783069
1215	50	1.9761904761904745
1215	51	1.9497354497354422
1212	1	-0.45767195767196256
1212	2	-5.550264550264558
1212	3	-2.407407407407412
1212	4	-1.0978835978836017
1212	5	2.142857142857146
1212	6	1.1375661375661252
1212	7	-1.4708994708994751
1212	8	1.275132275132279
1212	9	0.6719576719576636
1212	10	0.7460317460317469
1212	11	-2.1560846560846514
1212	12	-2.492063492063494
1212	13	1.0264550264550252
1212	14	1.6005291005291085
1212	15	1.216931216931222
1212	16	2.0899470899470884
1212	17	-0.6772486772486843
1212	18	1.187830687830683
1212	19	2.2089947089947017
1212	20	2.1190476190476204
1212	21	3.8915343915343925
1212	22	-0.9497354497354493
1212	23	-11.547619047619051
1212	24	1.8888888888888857
1212	25	2.941798941798936
1212	26	1.9021164021163983
1212	27	-9.222222222222221
1212	28	-9.25661375661376
1212	29	-3.5925925925925952
1212	30	-0.9179894179894106
1212	31	1.735449735449734
1212	32	1.301587301587304
1212	33	1.9814814814814738
1212	34	-10.698412698412703
1212	35	2.4126984126984112
1212	36	2.3042328042328037
1212	37	-2.661375661375665
1212	38	3.1904761904761827
1212	39	-9.679894179894177
1212	40	-0.05026455026455068
1212	41	-0.36507936507936734
1212	42	2.187830687830683
1212	43	-0.7857142857142918
1212	44	-0.9603174603174622
1212	45	-7.492063492063501
1212	46	-13.248677248677254
1212	47	-3.804232804232811
1212	48	-0.6931216931216966
1212	49	-3.68783068783069
1212	50	1.9761904761904745
1212	51	-1.0502645502645578
1213	1	2.5423280423280374
1213	2	-3.050264550264558
1213	3	3.092592592592588
1213	4	-4.097883597883602
1213	5	2.142857142857146
1213	6	1.1375661375661252
1213	7	1.5291005291005249
1213	8	1.275132275132279
1213	9	0.6719576719576636
1213	10	0.7460317460317469
1213	11	0.8439153439153486
1213	12	0.5079365079365061
1213	13	1.0264550264550252
1213	14	1.6005291005291085
1213	15	1.216931216931222
1213	16	3.0899470899470884
1213	17	-4.677248677248684
1213	18	-4.812169312169317
1213	19	-3.7910052910052983
1213	20	2.1190476190476204
1213	21	1.8915343915343925
1213	22	1.0502645502645507
1213	23	0.952380952380949
1213	24	2.8888888888888857
1213	25	1.941798941798936
1213	26	-4.097883597883602
1213	27	-7.722222222222221
1213	28	3.24338624338624
1213	29	1.9074074074074048
1213	30	2.0820105820105894
1213	31	-4.264550264550266
1213	32	1.301587301587304
1213	33	1.9814814814814738
1213	34	1.801587301587297
1213	35	-3.5873015873015888
1213	36	-3.6957671957671963
1213	37	-4.161375661375665
1213	38	-7.809523809523817
1213	39	-8.179894179894177
1213	40	2.9497354497354493
1213	41	2.6349206349206327
1213	42	-3.812169312169317
1213	43	2.214285714285708
1213	44	2.0396825396825378
1213	45	-4.992063492063501
1213	46	-3.7486772486772537
1213	47	2.195767195767189
1213	48	-3.6931216931216966
1213	49	-22.68783068783069
1213	50	-4.0238095238095255
1213	51	1.9497354497354422
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	0.0
23	2.0
24	5.0
25	9.0
26	9.0
27	22.0
28	26.0
29	38.0
30	36.0
31	62.0
32	58.0
33	69.0
34	78.0
35	103.0
36	121.0
37	162.0
38	237.0
39	262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.021555042340264	17.16705157813703	13.010007698229407	41.801385681293304
2	11.367127496159753	16.666666666666664	53.533026113671276	18.433179723502306
3	12.288786482334869	18.817204301075268	51.68970814132104	17.20430107526882
4	7.603686635944701	38.94009216589861	34.86943164362519	18.586789554531492
5	9.37019969278034	28.648233486943163	51.22887864823349	10.75268817204301
6	18.89400921658986	54.53149001536099	15.898617511520738	10.675883256528419
7	15.284178187403993	30.030721966205835	18.202764976958523	36.48233486943164
8	15.053763440860216	52.45775729646698	16.43625192012289	16.052227342549923
9	18.202764976958523	17.28110599078341	15.053763440860216	49.46236559139785
10	10.599078341013826	45.00768049155146	24.731182795698924	19.662058371735792
11	21.351766513056837	22.58064516129032	42.3963133640553	13.671274961597543
12	15.43778801843318	20.6605222734255	26.574500768049152	37.327188940092164
13	17.51152073732719	17.81874039938556	44.93087557603687	19.738863287250382
14	14.592933947772657	17.58832565284178	22.734254992319507	45.08448540706605
15	16.052227342549923	23.04147465437788	19.969278033794165	40.93701996927803
16	38.24884792626728	23.04147465437788	19.047619047619047	19.662058371735792
17	18.509984639016896	44.77726574500768	20.19969278033794	16.513056835637478
18	37.01996927803379	25.806451612903224	21.428571428571427	15.745007680491552
19	19.431643625192013	20.967741935483872	22.350230414746544	37.25038402457757
20	15.360983102918588	20.814132104454686	19.35483870967742	44.47004608294931
21	16.20583717357911	23.27188940092166	19.662058371735792	40.86021505376344
22	18.202764976958523	42.3195084485407	21.044546850998465	18.433179723502306
23	17.28110599078341	22.657450076804917	22.19662058371736	37.864823348694316
24	38.63287250384025	23.425499231950845	20.046082949308754	17.895545314900154
25	19.662058371735792	43.855606758832565	20.046082949308754	16.43625192012289
26	36.02150537634409	27.342549923195087	21.121351766513058	15.514592933947775
27	19.892473118279568	43.855606758832565	21.121351766513058	15.130568356374807
28	16.052227342549923	25.34562211981567	20.43010752688172	38.17204301075269
29	31.490015360983104	24.193548387096776	25.038402457757297	19.278033794162827
30	16.666666666666664	39.784946236559136	26.65130568356375	16.897081413210447
31	21.582181259600613	23.886328725038403	20.814132104454686	33.7173579109063
32	31.797235023041477	22.19662058371736	22.427035330261134	23.57910906298003
33	16.666666666666664	20.506912442396313	26.113671274961597	36.71274961597542
34	19.81566820276498	21.96620583717358	40.706605222734254	17.51152073732719
35	21.19815668202765	38.095238095238095	24.193548387096776	16.513056835637478
36	16.589861751152075	27.72657450076805	38.24884792626728	17.434715821812595
37	16.282642089093702	42.3963133640553	25.88325652841782	15.43778801843318
38	14.823348694316435	21.812596006144393	45.775729646697386	17.58832565284178
39	14.516129032258066	18.74039938556068	29.877112135176652	36.86635944700461
40	12.903225806451612	18.433179723502306	48.8479262672811	19.81566820276498
41	14.439324116743471	18.049155145929337	27.419354838709676	40.09216589861751
42	35.33026113671275	17.81874039938556	25.88325652841782	20.967741935483872
43	16.666666666666664	20.12288786482335	45.622119815668206	17.58832565284178
44	12.59600614439324	40.476190476190474	29.262672811059907	17.665130568356375
45	12.903225806451612	21.582181259600613	46.31336405529954	19.201228878648234
46	33.7173579109063	18.35637480798771	28.417818740399387	19.508448540706606
47	14.823348694316435	18.89400921658986	25.72964669738863	40.55299539170507
48	10.98310291858679	20.19969278033794	25.88325652841782	42.93394777265745
49	33.6405529953917	19.81566820276498	25.72964669738863	20.814132104454686
50	12.288786482334869	20.12288786482335	44.086021505376344	23.502304147465438
51	10.44546850998464	19.738863287250382	25.65284178187404	44.16282642089094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.0
24	1.0
25	1.0
26	3.5
27	6.0
28	8.5
29	11.0
30	24.0
31	37.0
32	43.5
33	50.0
34	70.0
35	90.0
36	105.5
37	121.0
38	113.5
39	106.0
40	107.5
41	109.0
42	95.5
43	82.0
44	91.0
45	100.0
46	87.0
47	74.0
48	66.0
49	58.0
50	71.0
51	84.0
52	194.0
53	304.0
54	161.0
55	18.0
56	16.5
57	15.0
58	10.5
59	6.0
60	5.5
61	5.0
62	5.0
63	5.0
64	5.0
65	5.0
66	4.5
67	4.0
68	2.5
69	1.0
70	1.5
71	2.0
72	2.5
73	3.0
74	2.5
75	1.0
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2304147465437788
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1302.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.95852534562212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.70259481037924	75.96006144393242
2	0.29940119760479045	0.4608294930875576
3	0.19960079840319359	0.4608294930875576
4	0.29940119760479045	0.9216589861751152
5	0.0	0.0
6	0.09980039920159679	0.4608294930875576
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.19960079840319359	2.227342549923195
>50	0.09980039920159679	4.224270353302612
>100	0.09980039920159679	15.284178187403993
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	199	15.284178187403993	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	55	4.224270353302612	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	17	1.30568356374808	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT	12	0.9216589861751152	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC	6	0.4608294930875576	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT	4	0.30721966205837176	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	4	0.30721966205837176	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	4	0.30721966205837176	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG	3	0.2304147465437788	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	3	0.2304147465437788	RNA PCR Primer, Index 27 (95% over 21bp)
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT	2	0.15360983102918588	No Hit
CTTATACACATCTCCGAGCCCCACGAGACGACGCTATATCTCGTATGCCGT	2	0.15360983102918588	No Hit
CTTATACACATCTCCGAGCCACGAGACGACGCTATATCTCGTATGCCGTCT	2	0.15360983102918588	RNA PCR Primer, Index 11 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.07680491551459294
23	0.0	0.0	0.07680491551459294
24	0.0	0.0	0.2304147465437788
25	0.0	0.0	0.30721966205837176
26	0.0	0.0	0.38402457757296465
27	0.0	0.0	0.38402457757296465
28	0.0	0.0	0.4608294930875576
29	0.0	0.0	1.075268817204301
30	0.0	0.0	1.7665130568356375
31	0.0	0.0	3.1490015360983103
32	0.0	0.0	4.377880184331797
33	0.0	0.0	5.376344086021505
34	0.0	0.0	6.221198156682028
35	0.0	0.0	6.912442396313364
36	0.0	0.0	8.525345622119815
37	0.0	0.0	10.291858678955453
38	0.0	0.0	12.288786482334869
39	0.0	0.0	14.439324116743471
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAC	40	1.5061232E-9	45.0	17
CTATATC	40	1.5061232E-9	45.0	33
ACGCTAT	40	1.5061232E-9	45.0	30
ATCTCGT	40	1.5061232E-9	45.0	37
ATCTCCG	40	1.5061232E-9	45.0	10
GCCCACG	40	1.5061232E-9	45.0	18
TATATCT	40	1.5061232E-9	45.0	34
CCACGAG	40	1.5061232E-9	45.0	20
GAGCCCA	40	1.5061232E-9	45.0	16
CACGAGA	40	1.5061232E-9	45.0	21
CCCACGA	40	1.5061232E-9	45.0	19
CGCTATA	40	1.5061232E-9	45.0	31
GCTATAT	40	1.5061232E-9	45.0	32
TCTCGTA	40	1.5061232E-9	45.0	38
GACGCTA	40	1.5061232E-9	45.0	29
ACGAGAC	35	3.817877E-8	45.0	22
TATCTCG	40	1.5061232E-9	45.0	36
AGACGAC	35	3.817877E-8	45.0	25
GACGACG	35	3.817877E-8	45.0	26
CGAGCCC	40	1.5061232E-9	45.0	15
>>END_MODULE