FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_42.34100000009b66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_42.34100000009b66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC19915.284178187403993No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC554.224270353302612No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT171.30568356374808No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT120.9216589861751152No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC60.4608294930875576No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT40.30721966205837176No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC40.30721966205837176No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC40.30721966205837176No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG30.2304147465437788No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC30.2304147465437788RNA PCR Primer, Index 27 (95% over 21bp)
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT20.15360983102918588No Hit
CTTATACACATCTCCGAGCCCCACGAGACGACGCTATATCTCGTATGCCGT20.15360983102918588No Hit
CTTATACACATCTCCGAGCCACGAGACGACGCTATATCTCGTATGCCGTCT20.15360983102918588RNA PCR Primer, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC401.5061232E-945.017
CTATATC401.5061232E-945.033
ACGCTAT401.5061232E-945.030
ATCTCGT401.5061232E-945.037
ATCTCCG401.5061232E-945.010
GCCCACG401.5061232E-945.018
TATATCT401.5061232E-945.034
CCACGAG401.5061232E-945.020
GAGCCCA401.5061232E-945.016
CACGAGA401.5061232E-945.021
CCCACGA401.5061232E-945.019
CGCTATA401.5061232E-945.031
GCTATAT401.5061232E-945.032
TCTCGTA401.5061232E-945.038
GACGCTA401.5061232E-945.029
ACGAGAC353.817877E-845.022
TATCTCG401.5061232E-945.036
AGACGAC353.817877E-845.025
GACGACG353.817877E-845.026
CGAGCCC401.5061232E-945.015
Produced by FastQC (version 0.11.2)