FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_41.34100000009b5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_41.34100000009b5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526912
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC50110.3281787031603655No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT15350.10052969653784893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7100.038.6608245
TGCGACC7800.032.01827629
GACTGCG7850.031.5277226
ATGCCGT8850.031.01602644
GCGACCT8000.030.93657730
TATGCCG8900.030.84177843
GCCCACG9050.030.57919918
CGACCTA8150.030.36719131
CCCACGA9350.029.8386819
CTGCGAC8300.029.81838828
AGCCCAC9500.029.1307117
CGCTATA852.0372681E-1029.11677731
ACTGCGA8550.028.94650727
GTATGCC9550.028.74259842
CGAGCCC9800.028.46853615
CCGAGCC9900.028.18097714
TATCTCG9650.027.97843736
CGTATGC9950.027.8132441
CCACGAG10200.027.35212520
TCGTATG10350.027.17310140
Produced by FastQC (version 0.11.2)