FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_40.34100000009b4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_40.34100000009b4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723978
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC110080.6385232294147605No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT17260.10011728687953093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13850.040.93733645
ATGCCGT16150.035.24656744
GCCCACG16100.035.21627818
CGTATGC16600.034.29108441
TATGCCG16650.034.0529843
CCCACGA16750.033.98419
AGCCCAC17350.032.6790817
CTCGTAT17650.032.50605439
GTATGCC17650.032.25110642
TCGTATG17750.032.19616740
CGAGCCC17850.032.01579315
CCGAGCC18250.031.19079614
TCTCGTA18450.030.85268438
ACGAGAC18850.030.55606522
ACCTTGC18100.030.33053227
CGAGACC18200.030.28750423
CCACGAG18950.030.0386320
CACGAGA19100.029.80272521
GAGCCCA19150.029.72491316
CGCTATA702.888919E-828.92765831
Produced by FastQC (version 0.11.2)