FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_4.34100000009840.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_4.34100000009840.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451560
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTATAGAACTCTAACTGCTTGTAAGGCTTGTAATTCAAGATCTATTTC8340.18469306404464522No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC5510.1220214367968819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.11781380104526532No Hit
CTATAGAACTCTAACTGCTTGTAAGGCTTGTAATTCAAGATCTATTTCACC4920.1089556205155461No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4880.1080698024625742No Hit
GTATAGTATATCTAGCGATAGTGAAGAGTAACGTGAGTGAAAGGTGAAAAG4730.10474798476392949No Hit
GTATAGATTCTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAAT4570.10120471255204182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCAA556.002665E-1140.90788318
TCGTATG801.0004442E-1030.93658640
ATGCCGT901.2732926E-1129.99911544
CTCGCGT300.0051447329.99911324
CTCGTAT803.3851393E-928.1241739
ACTCGCG406.161366E-428.1241712
GAACCTC1053.6379788E-1227.85632141
CGTATGC903.947207E-1027.49918741
TGGGGCA755.624861E-826.99920517
TATGCCG957.385097E-1026.05186343
CCCACGA957.385097E-1026.05186319
CCACGAG957.385097E-1026.05186320
CCTTGCA901.1872544E-824.99926228
TACCGCA450.001225612524.9992627
ACCTTGC1001.3405952E-924.7492727
CGACGTG551.3666805E-424.5447319
GCGATTG551.3666805E-424.54473117
TGCCGTC1301.8189894E-1224.23005345
GCAGAAC751.6942777E-623.99929419
AATCTCG851.8888932E-723.82282636