FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_39.34100000009b30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_39.34100000009b30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27770
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC7362.6503420957868205No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC4161.4980194454447247No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT1000.3601008282319049No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC740.2664746128916097No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT710.2556715880446525No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC390.14043932301044293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1450.043.44305445
ACGCTAT1100.040.9041830
CGACGCT1100.040.9041828
AGACGAC1050.040.70939625
GACGACG1050.040.70939626
ACGACGC1050.040.70939627
CGAGACG1050.040.70939623
GAGACGA1050.040.70939624
CGCTATA1150.039.12573231
GACGCTA1150.039.12573229
TATATCT1050.038.56679534
ATATCTC1050.038.56679535
GCTATAT1200.037.49549532
CTATATC1100.036.81376333
CCACGAG1900.036.7061220
CCCACGA1900.036.7061219
GCCCACG1850.036.48210518
CACGAGA1850.036.48210521
ACGAGAC1850.036.48210522
CGTATGC1850.036.48210541