##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_1_39.34100000009b30.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27770 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.47961829312207 38.0 38.0 38.0 32.0 38.0 2 36.67450486136118 38.0 38.0 38.0 32.0 38.0 3 36.674360821029886 38.0 38.0 38.0 32.0 38.0 4 36.73827871804105 38.0 38.0 38.0 32.0 38.0 5 36.79081742888009 38.0 38.0 38.0 32.0 38.0 6 38.690673388548795 40.0 38.0 40.0 38.0 40.0 7 38.489917176809506 40.0 38.0 40.0 38.0 40.0 8 38.46730284479654 40.0 38.0 40.0 38.0 40.0 9 38.53554195174649 40.0 38.0 40.0 38.0 40.0 10 38.502088584803744 40.0 38.0 40.0 38.0 40.0 11 38.60500540151242 40.0 38.0 40.0 38.0 40.0 12 38.47587324450846 40.0 38.0 40.0 38.0 40.0 13 38.52066978754051 40.0 38.0 40.0 38.0 40.0 14 38.464386028087866 40.0 38.0 40.0 38.0 40.0 15 38.45055815628376 40.0 38.0 40.0 38.0 40.0 16 38.40104429240187 40.0 38.0 40.0 38.0 40.0 17 38.39398631616853 40.0 38.0 40.0 38.0 40.0 18 38.361325171047895 40.0 38.0 40.0 38.0 40.0 19 38.384227583723444 40.0 38.0 40.0 38.0 40.0 20 38.3507742167807 40.0 38.0 40.0 38.0 40.0 21 38.40961469211379 40.0 38.0 40.0 38.0 40.0 22 38.44353619013324 40.0 38.0 40.0 38.0 40.0 23 38.47871804105149 40.0 38.0 40.0 38.0 40.0 24 38.41994958588405 40.0 38.0 40.0 38.0 40.0 25 38.35318689232985 40.0 38.0 40.0 38.0 40.0 26 38.330644580482534 40.0 38.0 40.0 38.0 40.0 27 38.28149081742888 40.0 38.0 40.0 38.0 40.0 28 38.27428880086424 40.0 38.0 40.0 38.0 40.0 29 38.277853799063735 40.0 38.0 40.0 38.0 40.0 30 38.272740367302845 40.0 38.0 40.0 38.0 40.0 31 38.29427439683111 40.0 38.0 40.0 38.0 40.0 32 38.24785740007202 40.0 38.0 40.0 38.0 40.0 33 38.27486496218941 40.0 38.0 40.0 38.0 40.0 34 38.2765574360821 40.0 38.0 40.0 38.0 40.0 35 38.265790421317966 40.0 38.0 40.0 38.0 40.0 36 38.244004321209935 40.0 38.0 40.0 38.0 40.0 37 38.12452286640259 40.0 38.0 40.0 38.0 40.0 38 38.151314368023044 40.0 38.0 40.0 38.0 40.0 39 37.98667626935542 40.0 38.0 40.0 38.0 40.0 40 38.08822470291682 40.0 38.0 40.0 38.0 40.0 41 38.06921137918617 40.0 38.0 40.0 38.0 40.0 42 38.049405833633415 40.0 38.0 40.0 38.0 40.0 43 38.0695714800144 40.0 38.0 40.0 38.0 40.0 44 38.01930140439323 40.0 38.0 40.0 38.0 40.0 45 37.983327331652866 40.0 38.0 40.0 38.0 40.0 46 37.891753691033486 40.0 38.0 40.0 32.0 40.0 47 37.88069859560677 40.0 38.0 40.0 32.0 40.0 48 37.803889088944906 40.0 38.0 40.0 32.0 40.0 49 37.72866402592726 40.0 38.0 40.0 32.0 40.0 50 37.73943104069139 40.0 38.0 40.0 32.0 40.0 51 37.34836154123155 40.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.3111518868503538 2201 2 -0.06276178143487954 2201 3 0.21217472246954117 2201 4 0.07501175993969156 2201 5 -0.35145627736319085 2201 6 -0.24312589057449685 2201 7 -0.06805370393067278 2201 8 0.3283450175683029 2201 9 0.27981857213050176 2201 10 0.0410929897426584 2201 11 -0.30285333600855324 2201 12 -0.12423868922049053 2201 13 -0.5677422401917838 2201 14 -0.5242595260845277 2201 15 -0.1335137965585247 2201 16 -1.0659097758901979 2201 17 -2.0354939059361143 2201 18 -0.3005482897191669 2201 19 0.24355830666716116 2201 20 0.33849772516420984 2201 21 -0.551595517095592 2201 22 0.6431544586494979 2201 23 -0.8570301336483936 2201 24 -0.5117671094617577 2201 25 -0.03637692916829138 2201 26 -0.5715684502875504 2201 27 -0.5641272187726827 2201 28 -0.5138936965582985 2201 29 -0.5807145326024923 2201 30 0.048158382988766846 2201 31 -0.031211970014517476 2201 32 -0.7905906477972522 2201 33 0.8164494048374493 2201 34 -0.18930929390016615 2201 35 -0.5492712410490412 2201 36 -0.7015149390175708 2201 37 0.21892866210131956 2201 38 -0.7045519818002433 2201 39 -0.01370959961025875 2201 40 -0.19797612864098824 2201 41 0.2688363563979479 2201 42 0.3815868373920992 2201 43 -0.11590474795858086 2201 44 -1.0182736247410773 2201 45 -0.4666067259810305 2201 46 -1.1577467296765818 2201 47 0.3741743049610946 2201 48 0.6204132619514624 2201 49 0.3768344614375323 2201 50 0.1481835513974019 2201 51 -0.8200111475265857 1101 1 0.13345153841829926 1101 2 0.26941011171262375 1101 3 -0.059602281014775826 1101 4 0.6086935253287749 1101 5 0.19616277025586015 1101 6 -0.03813169777542669 1101 7 0.7205641822481894 1101 8 0.44390831605842607 1101 9 0.8338255407716133 1101 10 0.44411273422581843 1101 11 -0.4410647181223766 1101 12 0.10049998673769522 1101 13 0.23365148803121372 1101 14 0.4246371057389311 1101 15 0.14058608729745714 1101 16 0.08798104873233115 1101 17 0.7026013321591265 1101 18 0.7714610018023222 1101 19 0.8440228827414913 1101 20 0.13524569264388475 1101 21 0.6876611611854742 1101 22 0.2291010324009477 1101 23 0.0848978570600849 1101 24 0.2913687790399848 1101 25 0.506596357707437 1101 26 0.8157718516868968 1101 27 0.3574755686837676 1101 28 0.2851771512930412 1101 29 0.605115897130375 1101 30 0.3019330635927133 1101 31 1.025117878998259 1101 32 0.36968809784735157 1101 33 0.26011955582467294 1101 34 0.5929206712566284 1101 35 1.0546776555827861 1101 36 0.8490077090660577 1101 37 0.47270334270526604 1101 38 1.1386535931126502 1101 39 1.4229919102619846 1101 40 0.7509205031824706 1101 41 0.42505006139214174 1101 42 0.07322446805412142 1101 43 0.8846759721343318 1101 44 0.05547782705914983 1101 45 0.5728822403372007 1101 46 -0.09096391899133494 1101 47 0.6267875453792158 1101 48 0.11809038157980467 1101 49 -0.3293211715473703 1101 50 0.5088107290977533 1101 51 -0.2944594634383222 2202 1 0.16829474399320787 2202 2 0.19914298046987966 2202 3 0.37884138913621257 2202 4 -0.06784538291744724 2202 5 -0.23240865831557045 2202 6 -0.14788779533640195 2202 7 -0.5442441801211544 2202 8 0.6616783509016315 2202 9 -0.5773242850123594 2202 10 0.6601406087902788 2202 11 -0.040948574103794044 2202 12 0.42338035839855337 2202 13 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0.35503186532668707 1213 4 0.44405937898731196 1213 5 0.8211627702558602 1213 6 0.539612204663598 1213 7 1.2444462960693272 1213 8 0.9235831128063978 1213 9 0.8988661911781222 1213 10 0.7672834659331329 1213 11 0.9471466639914468 1213 12 1.2448089298271299 1213 13 1.2417815693320264 1213 14 0.9043119024869029 1213 15 1.1700576320128988 1213 16 0.9162330812526562 1213 17 0.7234346654924622 1213 18 1.2054040912332198 1213 19 1.291177354286205 1213 20 1.1301643918308812 1213 21 1.1805473400472621 1213 22 1.0776782681733081 1213 23 1.273922247303986 1213 24 1.3929947953001474 1213 25 1.3683849755936137 1213 26 0.40164583542673427 1213 27 1.2334918288463683 1213 28 1.2242015415369423 1213 29 1.2169045150165516 1213 30 1.0183964782268617 1213 31 1.117597553795008 1213 32 1.0546474474408427 1213 33 1.1259732143612595 1213 34 0.6767621346712644 1213 35 1.0429906637128639 1213 36 1.2062231562205241 1213 37 0.9600000906727502 1213 38 1.182352780104523 1213 39 1.3731951622945004 1213 40 1.251428633263771 1213 41 1.2390744516360428 1213 42 1.6077773135825808 1213 43 1.276952394898558 1213 44 1.4579168514493972 1213 45 1.3726789883046848 1213 46 1.5624913655615131 1213 47 1.6003647811515762 1213 48 1.7156513571895573 1213 49 1.912548747151817 1213 50 1.3416359323497815 1213 51 1.5490364715210276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 2.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 12.0 23 16.0 24 36.0 25 66.0 26 83.0 27 135.0 28 188.0 29 279.0 30 317.0 31 426.0 32 527.0 33 574.0 34 732.0 35 930.0 36 1206.0 37 1855.0 38 3624.0 39 16758.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.981896209744306 20.343322874968443 13.851923978506257 27.822856936780987 2 13.662225423118473 22.283039250990278 43.88908894490457 20.165646380986676 3 15.599567879006122 26.25135037810587 36.21534029528268 21.93374144760533 4 11.850918257111992 23.122074180770618 38.78646020885848 26.240547353258915 5 12.315448325531149 37.00036010082823 38.70363701836514 11.980554555275477 6 25.243068059056533 44.16996759092546 18.32553114872164 12.261433201296363 7 23.230104429240185 33.500180050414116 22.92041771696075 20.349297803384946 8 20.72380266474613 41.98055455527548 20.95066618653223 16.344976593446166 9 23.359740727403672 17.518905293482177 21.375585163845876 37.74576881526828 10 13.86388188692834 33.09326611451206 31.08390349297803 21.958948505581564 11 28.844076341375587 25.53474972992438 29.38782859200576 16.233345336694274 12 18.764854159164564 26.150522146200938 31.67446885127836 23.41015484335614 13 23.997119193374143 22.211019085343896 32.82319049333813 20.968671227943826 14 18.768455167446884 21.825711199135757 28.32193014043932 31.08390349297803 15 21.004681310767015 28.764854159164564 24.195174648901695 26.03528988116673 16 24.270795822830394 29.27979834353619 25.740007202016564 20.709398631616853 17 20.205257472092185 33.52898811667267 26.579042131796903 19.686712279438243 18 25.023406553835077 29.002520705797625 27.191213539791143 18.78285920057616 19 21.418797263233706 28.051854519265397 26.942743968311127 23.586604249189776 20 20.44652502700756 26.77349657904213 26.89593086064098 25.884047533309328 21 20.468131076701475 26.982355059416633 27.389268995318687 25.160244868563197 22 20.111631256751892 31.541231544832556 27.410875045012606 20.936262153402954 23 19.989196975153043 28.127475693194093 27.144400432120996 24.73892689953187 24 24.33201296362982 28.095066618653224 27.709758732445085 19.863161685271876 25 20.691393590205255 32.117392870003606 27.33885487936622 19.85235866042492 26 24.875765214259992 28.33993518185092 26.996759092545915 19.787540511343177 27 20.421317969031332 32.67554915376306 27.191213539791143 19.71191933741448 28 19.84155563557796 28.07706157724163 27.08318329132157 24.99819949585884 29 22.59992797983435 28.192293842275838 28.354339214980197 20.853438962909614 30 20.270075621173927 31.152322650342096 28.685631976953545 19.89196975153043 31 21.840115232265035 27.104789341015483 27.626935541951745 23.428159884767734 32 23.568599207778178 27.292041771696073 27.23802664746129 21.90133237306446 33 20.129636298163486 26.838314728123873 29.481454807346058 23.550594166366583 34 20.709398631616853 27.500900252070583 31.235145840835433 20.554555275477135 35 21.71407994238387 29.560676989557077 28.087864602088587 20.63737846597047 36 19.564277997839394 29.002520705797625 30.777817788980915 20.655383507382066 37 19.715520345696795 32.27223622614332 27.861001080302483 20.1512423478574 38 19.14296002880807 27.065178249909977 33.03925099027728 20.75261073100468 39 19.449045732805185 26.197335253871085 29.096146921137915 25.25747209218581 40 19.189773136478212 25.79042131796903 33.3273316528628 21.692473892689954 41 19.715520345696795 26.10010803024847 28.995318689232985 25.18905293482175 42 23.889088944904575 25.851638458768456 28.703637018365143 21.55563557796183 43 20.02160604969391 25.556355779618293 33.28411955347497 21.13791861721282 44 18.577601728483977 31.18113071660065 28.991717680950668 21.24954987396471 45 18.293122074180772 26.982355059416633 33.56859920777818 21.155923658624413 46 23.802664746128915 25.86964350018005 28.973712639539073 21.353979114151965 47 18.79726323370544 26.21534029528268 28.498379546272957 26.489016924738927 48 18.343536190133236 26.082102988836876 28.11307166006482 27.46128916096507 49 23.25531148721642 25.779618293122077 28.923298523586606 22.041771696074903 50 18.617212819589486 25.79042131796903 33.33093266114512 22.261433201296363 51 18.120273676629456 26.01368383147281 28.793662225423116 27.07238026647461 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.5 6 3.0 7 2.0 8 1.0 9 2.0 10 3.0 11 5.0 12 7.0 13 22.0 14 37.0 15 59.0 16 81.0 17 84.0 18 87.0 19 74.5 20 62.0 21 68.0 22 74.0 23 86.5 24 99.0 25 146.0 26 276.0 27 359.0 28 501.0 29 643.0 30 842.5 31 1042.0 32 1315.5 33 1589.0 34 1899.5 35 2210.0 36 2371.0 37 2532.0 38 2653.5 39 2775.0 40 2643.0 41 2511.0 42 2395.5 43 2280.0 44 2012.5 45 1745.0 46 1644.5 47 1544.0 48 1424.0 49 1304.0 50 1347.5 51 1391.0 52 1805.0 53 2219.0 54 1511.0 55 803.0 56 642.0 57 481.0 58 440.5 59 400.0 60 340.0 61 280.0 62 240.5 63 201.0 64 180.0 65 159.0 66 184.5 67 210.0 68 165.5 69 121.0 70 131.5 71 142.0 72 118.5 73 95.0 74 74.0 75 31.0 76 9.0 77 10.5 78 12.0 79 12.0 80 12.0 81 6.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14764133957508102 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 27770.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.06566422783469 #Duplication Level Percentage of deduplicated Percentage of total 1 96.4363718403306 85.89169513681496 2 2.401898463741572 4.278533641619177 3 0.6069200386195028 1.621672091184873 4 0.1936547327676127 0.68991949619245 5 0.11063560456413843 0.49269168038765243 6 0.08204991694621287 0.4384698211591871 7 0.02200487409168338 0.13719151090579557 8 0.03869680152914794 0.2757245065349 9 0.021165528011061956 0.16966096299342664 >10 0.06611858524195316 0.9071047106259635 >50 0.012290168493923063 0.8939494833129209 >100 0.00409672283130474 1.5178897349303677 >500 0.00409672283130474 2.6854972233383423 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 736 2.6503420957868205 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 416 1.4980194454447247 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT 100 0.3601008282319049 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 74 0.2664746128916097 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT 71 0.2556715880446525 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 39 0.14043932301044293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0036010082823190494 4 0.0 0.0 0.0036010082823190494 5 0.0 0.0 0.007202016564638099 6 0.0 0.0 0.007202016564638099 7 0.0 0.0 0.007202016564638099 8 0.0 0.0 0.007202016564638099 9 0.0 0.0 0.007202016564638099 10 0.0 0.0 0.007202016564638099 11 0.0 0.0 0.007202016564638099 12 0.0 0.0 0.007202016564638099 13 0.0 0.0 0.007202016564638099 14 0.0 0.0 0.007202016564638099 15 0.0 0.0 0.007202016564638099 16 0.0 0.0 0.007202016564638099 17 0.0 0.0 0.010803024846957148 18 0.0 0.0 0.010803024846957148 19 0.0 0.0 0.010803024846957148 20 0.0 0.0 0.010803024846957148 21 0.0 0.0 0.010803024846957148 22 0.0 0.0 0.010803024846957148 23 0.0 0.0 0.025207057976233346 24 0.0 0.0 0.03240907454087144 25 0.0 0.0 0.04321209938782859 26 0.0 0.0 0.06841915736406194 27 0.0 0.0 0.08282319049333814 28 0.0 0.0 0.12603528988116672 29 0.0 0.0 0.21966150522146202 30 0.0 0.0 0.3781058696435002 31 0.0 0.0 0.6985956067698955 32 0.0 0.0 0.9722722362261433 33 0.0 0.0 1.303564998199496 34 0.0 0.0 1.6600648181490818 35 0.0 0.0 1.9949585884047534 36 0.0 0.0 2.484695714800144 37 0.0 0.0 3.0464530068419156 38 0.0 0.0 3.817068779258192 39 0.0 0.0 4.645300684191573 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 145 0.0 43.443054 45 ACGCTAT 110 0.0 40.90418 30 CGACGCT 110 0.0 40.90418 28 AGACGAC 105 0.0 40.709396 25 GACGACG 105 0.0 40.709396 26 ACGACGC 105 0.0 40.709396 27 CGAGACG 105 0.0 40.709396 23 GAGACGA 105 0.0 40.709396 24 CGCTATA 115 0.0 39.125732 31 GACGCTA 115 0.0 39.125732 29 TATATCT 105 0.0 38.566795 34 ATATCTC 105 0.0 38.566795 35 GCTATAT 120 0.0 37.495495 32 CTATATC 110 0.0 36.813763 33 CCACGAG 190 0.0 36.70612 20 CCCACGA 190 0.0 36.70612 19 GCCCACG 185 0.0 36.482105 18 CACGAGA 185 0.0 36.482105 21 ACGAGAC 185 0.0 36.482105 22 CGTATGC 185 0.0 36.482105 41 >>END_MODULE