FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_38.34100000009b23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_38.34100000009b23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48819
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC8431.7267867018988508No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC5831.1942071734365718No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT1380.2826768266453635No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT940.19254798336713166No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA660.13519326491734776No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT650.13314488211556974No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAT900.039.99726530
GACGCTA900.039.99726529
GAGACGA1000.038.24738724
CGAGACG1000.038.24738723
TGCCGTC1600.037.96615645
TATATCT950.037.89214734
CGCTATA950.037.89214731
CCACAAG250.002088140735.997543
CGACCAG250.002088140735.9975418
AGACGAC1000.035.9975425
TATCTCG1150.035.2149936
ATATCTC1050.034.2833735
GACGACG1050.034.2833726
ACGACGC1050.034.2833727
ATGCCGT1800.033.74769244
CCACGAG1950.033.4592520
CGACGCT1100.032.72503728
CTATATC1100.032.72503733
ATCTCCG2000.032.62277210
GCCCACG2000.032.62277218
Produced by FastQC (version 0.11.2)