FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_37.34100000009b09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_37.34100000009b09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613888
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10360.16876042535446206No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC8930.14546627397831527No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC6960.11337572977481235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA458.693987E-734.99854331
ATGCCGT1450.032.58485444
GCCCACG1500.031.4986918
GTATGCC1500.031.4986942
TATGCCG1550.030.48260143
CTAGTAT300.005146458729.998753
CTCGTAT1650.028.63517239
CGTATGC1700.027.7929641
ATTCGGC1500.025.4989432
TATCTCG1500.025.4989436
ACGTTAG801.03360435E-725.3589531
CGAGACG1350.024.99895923
CACGAGA1950.024.2297621
CCCACGA1950.024.2297619
GGGGCAA2900.024.05072418
TACCCGG2900.024.0507246
CGGGGCA3500.023.78472317
TCGTATG2000.023.62401640
ACCTATA2800.023.3463381
CGGCAGG1750.023.14189335
Produced by FastQC (version 0.11.2)