FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_36.34100000009afb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_36.34100000009afb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544148
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC11530.21189088262752045No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG6840.12570109602534604No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC6000.110264119320479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1600.036.56152743
ATGCCGT1700.035.73434444
GCCCACG1700.034.4108518
GTATGCC1850.032.83696742
CGTATGC1900.031.97283641
TCGTATG1850.031.62078340
CCCACGA2000.030.37419319
TAGACCG300.00514557129.9992055
CCGACTG406.1628956E-428.12425229
CTCGTAT2150.027.2085839
GAGACCC2000.026.99928324
CTCCGAG2200.026.59020212
AGACCCT2150.025.11561225
CCGAGCC2250.024.99933814
TCGACAC450.00122591424.9993363
TCTCGTA2400.024.37435338
ACCCTGG2250.023.99936527
CGAGACC2350.023.93553423
TGGATAA2300.023.47763831
GGATAAT2350.022.97811332
Produced by FastQC (version 0.11.2)