FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_35.34100000009aee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_35.34100000009aee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287765
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC57960.4500821190201628No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT17870.13876755463923932No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA14010.1087931416058054No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6650.037.8934345
ATGCCGT8250.030.2716844
AGCGCAA300.00514835629.99896245
TATGCCG8450.029.55519343
CCCACGA8750.028.79900619
CGCCACG554.16657E-628.63537420
CGTATGC8700.028.44729441
GCCACGT654.8756374E-727.69135321
GCCCACG9100.027.6913518
GTATGCC9050.027.59573242
CCACGAG9500.026.52539820
TATCTCG9600.026.4834636
CGAGACA9250.026.26936523
CGCGCGC608.194747E-626.24909210
CTCGTAT9550.026.1509339
ACGAGAC9550.026.1509322
TCGTATG9550.025.91533740
TCTCGTA10050.024.8498938
CACGAGA10100.024.7268721
GTCGCGC651.5243433E-524.2299338
Produced by FastQC (version 0.11.2)